Photochemical micropatterning of carbohydrates on a surface
Photochemical micropatterning of carbohydrates on a surface
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DOI：
10.1021/la0531042
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发表时间：
2006-03-14
期刊：
影响因子：
3.9
通讯作者：
Koberstein, JT
中科院分区：
文献类型：
--
作者：
Carroll, GT;Wang, DN;Koberstein, JT
在这份报告中，我们展示了一种通用的方法将未经修饰的碳水化合物固定和图案化到玻璃基板上。该方法利用一种新颖的自组装单分子膜在空气-单分子膜界面呈现光活性邻苯二甲酰亚胺发色团。在紫外光照射下，邻苯二甲酰亚胺末端基团接枝到表面吸附的碳水化合物上，推测是通过氢吸收机制，然后自由基重组形成共价键。固定化的碳水化合物薄膜通过荧光、椭偏仪和接触角测量得到了证明。单糖、寡糖和多糖的表面微图案是通过接触光掩模曝光产生的，并通过将水冷凝到表面上来可视化。共价偶联的效率取决于表面的热力学状态。当碳水化合物的表面相互作用通过在单层中掺入端胺分子而增加时，表面接枝的碳水化合物的量增加。用这种性质的混合单层修饰的玻璃基板被用来构建碳水化合物微阵列，方法是用机器人发现碳水化合物，然后用紫外光照射它们，使碳水化合物共价连接。表面固定的多糖显示了明确的抗体识别的抗原决定簇。因此，我们证明，这项新技术结合了使用当前最先进的机器人微点技术创建碳水化合物微阵列的能力，以及控制固定碳水化合物图案形状的能力，其空间分辨率由UV波长定义，形状由光掩模定义。
In this report, we demonstrate a versatile method for the immobilization and patterning of unmodified carbohydrates onto glass substrates. The method employs a novel self-assembled monolayer to present photoactive phthalimide chromophores at the air-monolayer interface. Upon exposure to UV radiation, the phthalimide end-groups graft to surface-adsorbed carbohydrates, presumably by a hydrogen abstraction mechanism followed by radical recombination to form a covalent bond. Immobilized carbohydrate thin films are evidenced by fluorescence, ellipsometry and contact-angle measurements. Surface micropatterns of mono-, oligo-, and polysaccharides are generated by exposure through a contact photomask and are visualized by condensing water onto the surface. The efficiency of covalent coupling is dependent on the thermodynamic state of the surface. The amount of surface-grafted carbohydrate is enhanced when carbohydrate surface interactions are increased by the incorporation of amine-terminated molecules into the monolayer. Glass substrates modified with mixed monolayers of this nature are used to construct carbohydrate microarrays by spotting the carbohydrates with a robot and subsequently illuminating them with UV light to covalently link the carbohydrates. Surface-immobilized polysaccharides display well-defined antigenic determinants for antibody recognition. We demonstrate, therefore, that this novel technology combines the ability to create carbohydrate microarrays using the current state-of-the-art technology of robotic microspotting and the ability to control the shape of immobilized carbohydrate patterns with a spatial resolution defined by the UV wavelength and a shape defined by a photomask.