Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM
Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM
复制标题
DOI：
10.1073/pnas.1604693113
复制
发表时间：
2016-09-13
影响因子：
11.1
通讯作者：
Jensen, Grant J.
中科院分区：
文献类型：
--
作者：
Briegel, Ariane;Ortega, Davi R.;Jensen, Grant J.
几乎所有能动的细菌细胞都使用高度敏感和适应性强的感觉系统来检测环境中营养物质浓度的变化，并引导它们朝向引诱剂和远离驱避剂的运动。研究最多的细菌化学感受器阵列是膜结合的。许多运动细菌含有一个或多个额外的（有时是纯细胞质的）化学感受器系统。霍乱弧菌包含三个趋化性簇（I，II和III）。在这里，我们使用电子冷冻断层扫描，探索霍乱弧菌的细胞质化学感受器阵列，并确定它是由来自簇I的蛋白质形成的。我们进一步确定了一个化学受体与一个不寻常的结构域架构，DosM，这是必不可少的细胞质阵列的形成。DosM包含两个信号结构域，并跨越两层细胞质阵列。最后，我们提出的证据表明，这种类型的受体是重要的细胞质阵列的结构稳定性。
Nearly all motile bacterial cells use a highly sensitive and adaptable sensory system to detect changes in nutrient concentrations in the environment and guide their movements toward attractants and away from repellents. The best-studied bacterial chemoreceptor arrays are membrane-bound. Many motile bacteria contain one or more additional, sometimes purely cytoplasmic, chemoreceptor systems. Vibrio cholerae contains three chemotaxis clusters (I, II, and III). Here, using electron cryotomography, we explore V. cholerae's cytoplasmic chemoreceptor array and establish that it is formed by proteins from cluster I. We further identify a chemoreceptor with an unusual domain architecture, DosM, which is essential for formation of the cytoplasmic arrays. DosM contains two signaling domains and spans the two-layered cytoplasmic arrays. Finally, we present evidence suggesting that this type of receptor is important for the structural stability of the cytoplasmic array.