DVD diagnostic software for reading disc-based bioassays, a comparative study
DVD diagnostic software for reading disc-based bioassays, a comparative study
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DOI：
10.1016/j.snb.2013.12.087
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发表时间：
2014-05
影响因子：
8.4
通讯作者：
Xue-Jiao Zhao;Xiaochun Li;C. Cui;Hua-Zhong Yu
中科院分区：
文献类型：
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作者：
Xue-Jiao Zhao;Xiaochun Li;C. Cui;Hua-Zhong Yu
基于光盘（CD）技术的生物测定已被开发为新型即时护理（POC）工具，可用于化学分析和生物医学诊断的各种应用。在此，我们研究了使用带有免费 DVD 诊断软件的普通台式/笔记本电脑的标准光盘驱动器筛选基于光盘的生物测定的准确性。特别是，我们比较了三个免费在线 DVD 诊断软件程序：PlexUtilities、Kprobe 和 Optical Drive Control (ODC) 的性能，首先使用毫米大小的墨点阵列，然后使用生物素-链霉亲和素结合测定。我们已经证实，PIF（奇偶校验内部故障）数量与墨点的大小有关，而PIF密度（每单位面积的集成PIF计数）与墨点的光学暗度比（ODR）成正比。在标准 DVD-R 上制备的生物素-链霉亲和素结合测定的读数结果进一步证明了所有三种类型的光盘诊断软件程序的定量准确性。
Compact disc (CD) technology-based bioassays have been developed as novel point-of-care (POC) tools for various applications in chemical analysis and biomedical diagnosis. Herein we investigate the accuracy of screening disc-based bioassays with standard optical drives of ordinary desktop/notebook computers with free DVD diagnostic software. In particular, we have compared the performance of three free online DVD diagnostic software programs: PlexUtilities, Kprobe and Optical Drive Control (ODC), first with arrays of mm-size ink dots and then with biotin–streptavidin binding assays. We have confirmed that the PIF (parity inner failure) number is related to the size of the ink spots, while the PIF density (integrated PIF counts per unit area) is proportional to the optical darkness ratio (ODR) of the spots. The reading results with a biotin–streptavidin binding assay prepared on a standard DVD-R further prove the accuracy of quantitation with all three types of disc diagnostic software programs.