The electric field near human skin wounds declines with age and provides a noninvasive indicator of wound healing.
The electric field near human skin wounds declines with age and provides a noninvasive indicator of wound healing.
复制标题
DOI：
10.1111/j.1524-475x.2011.00723.x
复制
发表时间：
2011-09
期刊：
影响因子：
--
通讯作者：
Lui K
中科院分区：
文献类型：
--
作者：
Nuccitelli R;Nuccitelli P;Li C;Narsing S;Pariser DM;Lui K
由于15-50 mV的经皮电位（内部为正），所有人体皮肤伤口都会产生损伤电流。该电流的流动在表皮内产生横向电场，该横向电场在伤口边缘处比在距离伤口边缘更侧向的区域处更负。该区域中的电场可高达40 mV/mm 2，并且已显示该量级的电场刺激人角质形成细胞向受伤区域迁移。在流出伤口后，电流通过表皮和角质层之间的空间返回，在表皮上方产生相反方向的横向场。在这里，我们报告的结果，从第一个临床试验，旨在测量这种横向电场邻近人体皮肤伤口非侵入性。使用一种新的仪器Dermacorder®，我们发现18-25奥尔兹人中表皮和邻近柳叶刀伤口的角质层之间的空间中的平均横向电场为107-148 mV/mm，比65-80奥尔兹的人平均大48%。我们还进行了广泛的测量的横向电场附近的小鼠伤口愈合，并通过伤口的组织切片，以确定电场和上皮伤口闭合率之间的相关性，比较这个字段。创伤后即刻的平均横向电场为122 ± 9 mV/mm。当伤口填充有厚的、无组织的表皮层时，平均电场福尔斯降至79 ± 4 mV/mm。一旦该表皮形成仅具有三个细胞层的紧凑结构，平均电场为59 ± 5 mV/mm。因此，表面电位的峰-峰空间变化在新鲜伤口中最大，并且随着伤口闭合而缓慢下降。当伤口如预期保持湿润时，伤口愈合的速率略高，但我们发现电场的幅度与伤口愈合的速率之间没有相关性。
Due to the transepidermal potential of 15-50 mV, inside positive, an injury current is driven out of all human skin wounds. The flow of this current generates a lateral electric field within the epidermis that is more negative at the wound edge than at regions more lateral from the wound edge. Electric fields in this region could be as large as 40 mV/mm2, and electric fields of this magnitude have been shown to stimulate human keratinocyte migration toward the wounded region. After flowing out of the wound, the current returns through the space between the epidermis and stratum corneum, generating a lateral field above the epidermis in the opposite direction. Here we report the results from the first clinical trial designed to measure this lateral electric field adjacent to human skin wounds non-invasively. Using a new instrument, the Dermacorder®, we found that the mean lateral electric field in the space between the epidermis and stratum corneum adjacent to a lancet wound in 18-25 year olds is 107-148 mV/mm, 48% larger on average than that in 65-80 year olds. We also conducted extensive measurements of the lateral electric field adjacent to mouse wounds as they healed and compared this field with histological sections through the wound to determine the correlation between the electric field and the rate of epithelial wound closure. Immediately after wounding the average lateral electric field was 122 ± 9 mV/mm. When the wound is filled in with a thick, disorganized epidermal layer, the mean field falls to 79 ± 4 mV/mm. Once this epidermis forms a compact structure with only three cell layers, the mean field is 59 ± 5 mV/mm. Thus, the peak-to-peak spatial variation in surface potential is largest in fresh wounds and slowly declines as the wound closes. The rate of wound healing is slightly greater when wounds are kept moist as expected but we could find no correlation between the amplitude of the electric field and the rate of wound healing.