Diagnostic protein marker patterns in squamous cervical cancer
Diagnostic protein marker patterns in squamous cervical cancer
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DOI：
10.1002/prca.200900086
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Auer, Gert
中科院分区：
文献类型：
--
作者：
Lomnytska, Marta I.;Becker, Susanne;Auer, Gert
目的：宫颈癌是女性第二大常见恶性肿瘤。我们的目的是确定额外的标志物蛋白质模式的客观诊断宫颈鳞状细胞癌（SCC）。实验设计：收集组织活检SCC，鳞状阴道癌（SVC），正常宫颈和阴道粘膜进行2-DE，SameSpot分析，MALDI-TOF-MS蛋白鉴定，并通过免疫组化分析所选蛋白质的表达。在通过表达差异选择的148个蛋白质点中，鉴定了99个蛋白质。SCC的差异蛋白质模式是例如过表达（OE）真核翻译起始因子3-2 β、中性粒细胞胞质因子2、膜联蛋白A6（ANXA 6），SVC是OE组织蛋白酶D、γ-连环蛋白、RAB 2A，两种癌症都是OE载脂蛋白E、原肌球蛋白3、HSPA 8和低表达细胞角蛋白13、骨甘氨酸。在SCC中，观察到中性粒细胞胞浆因子2、PRDX 2、HSP 27（9/10例）、ANXA 6（9/10例）的核表达，而原肌球蛋白4仅在2/10例中表达。81.1%（43/53）的蛋白质斑点表达与蛋白质免疫表达一致（www.proteinatlas.org）。结论和临床相关性：SCC的特征在于特异性组织标志物蛋白质模式，其允许客观检测疾病。它们可以成为客观的自动化细胞学筛查的基础，并改善目前SCC的诊断。
Purpose: Cervical cancer is the second most prevalent malignancy of women. Our aim was to identify additional marker protein patterns for objective diagnosis of squamous cervical cancer (SCC).Experimental design: Collected tissue biopsies of SCC, squamous vaginal cancer (SVC), normal cervical and vaginal mucosa were subjected to 2-DE, SameSpot analysis, MALDI-TOF-MS protein identification, and analysis of the expression of selected proteins by immunohistochemistry.Results: In 148 protein spots selected by the difference in expression 99 proteins were identified. A differential protein pattern for SCC was, e.g. over-expressed (OE) eukaryotic translation initiation factor 3-2 beta, neutrophil cytosolic factor 2, annexin A6 (ANXA6), for SVC it was OE cathepsin D, gamma-catenin, RAB2A, for both cancers it was OE apolipoprotein E, tropomyosin 3, HSPA8, and underexpressed cytokeratin 13, osteoglycin. In SCC nuclear expression of neutrophil cytosolic factor 2, PRDX2, HSP27 (nine of ten cases), ANXA6 (nine of ten cases) was observed while tropomyosin 4 was expressed only in two of ten cases. There was 81.1% (43/53) agreement between the expression of protein spots and the immune expression of proteins (www.proteinatlas.org).Conclusions and clinical relevance: SCC is characterized by specific tissue marker protein patterns that allow objective detection of the disease. They can become a basis for objective automated cytology-based screening and improve current diagnostics of SCC.