Poly(ethylene glycol)-lipase complexes that are highly active and enantioselective in ionic liquids.
Poly(ethylene glycol)-lipase complexes that are highly active and enantioselective in ionic liquids.
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聚乙二醇-脂肪酶复合物在离子液体中具有高活性和对映选择性。
DOI：
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发表时间：
2004
影响因子：
3.2
通讯作者：
M. Goto
中科院分区：
文献类型：
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作者：
T. Maruyama;H. Yamamura;Takahiro Kotani;N. Kamiya;M. Goto
研究了脂肪酶催化的二烷基咪唑离子液体中乙酸乙烯酯与2-苯基-1-丙醇的醇解反应。虽然天然脂肪酶粉在离子液体中表现出很低的活性，但聚乙二醇-脂肪酶复合物的形成提高了离子液体中脂肪酶的活性。peg -脂肪酶复合物在离子液体中的活性高于在普通有机溶剂（正己烷、异辛烷和二甲亚砜）中的活性。用4-氨基苯酞酰亚胺进行荧光测量，结果表明离子液体比用于非水酶学的有机溶剂更亲水。脂肪酶催化离子液体（[Bmim][PF6]）醇解的动力学研究表明，离子液体中2-苯基-1-丙醇的米切里斯常数（Km）是正己烷中的一半，表明离子液体稳定了酶-底物络合物。最后，我们利用peg -脂肪酶配合物对离子液体中的1-苯乙醇进行了对映选择性醇解，获得了与正己烷相当的高对映选择性。
Lipase-catalyzed alcoholysis between vinyl acetate and 2-phenyl-1-propanol was investigated in dialkylimidazolium-based ionic liquids. Although native lipase powder exhibited very low activity in an ionic liquid, forming a poly(ethylene glycol)(PEG)-lipase complex improved the lipase activity in the ionic liquid. The activity of the PEG-lipase complex was higher in ionic liquids than in common organic solvents (n-hexane, isooctane and dimethylsulfoxide). Fluorescence measurements using 4-aminophthalimide revealed that the ionic liquids were more hydrophilic than the organic solvents used for non-aqueous enzymology. A kinetic study of lipase-catalyzed alcoholysis in an ionic liquid ([Bmim][PF6]) revealed that the Michaelis constant (Km) for 2-phenyl-1-propanol in the ionic liquid was half that in n-hexane, suggesting that the ionic liquid stabilized the enzyme-substrate complex. Finally, we carried out enantioselective alcoholysis of 1-phenylethanol in ionic liquids employing the PEG-lipase complex, and obtained high enantioselectivity, comparable to that in n-hexane.