Optimizing the bio-degradability and biocompatibility of a biogenic collagen membrane through cross-linking and zinc-doped hydroxyapatite
Optimizing the bio-degradability and biocompatibility of a biogenic collagen membrane through cross-linking and zinc-doped hydroxyapatite
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通过交联和锌掺杂羟基磷灰石优化生物胶原膜的生物降解性和生物相容性
DOI：
10.1016/j.actbio.2022.02.004
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Zhuofan Chen
中科院分区：
文献类型：
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作者：
You Wu;Shoucheng Chen;Pu Luo;Shudan Deng;Zhengjie Shan;Jinghan Fang;Xingchen Liu;Jiaxin Xie;Runheng Liu;Shiyu Wu;Xiayi Wu;Zetao Chen;Kelvin W.K. Yeung;Quan Liu;Zhuofan Chen
生物胶原膜已被广泛用作引导骨再生（GBR）和引导组织再生（GTR）中的软组织屏障。然而，它们的临床性能仍然不令人满意，因为它们的机械强度低和体内降解速率快。虽然用化学试剂交联对于延长胶原膜的降解时间是有效和可靠的，但是在该过程中已经观察到一些不利影响，包括潜在的细胞毒性和不期望的组织整合。锌作为一种重要的营养微量元素，在促进细胞生长和调节胶原基质降解方面起着积极的作用。在此，生物胶原膜与戊二醛-阿仑膦酸钠交联，以延长其降解时间。通过掺入掺锌纳米羟基磷灰石（nZnHA），增强了胶原蛋白的理化和生物学特性，保留了胶原蛋白的天然结构。具体而言，交联结合1%和2%nZnHA的掺入似乎赋予膜在交联膜中最适当的生物相容性和组织整合能力，以及在大鼠皮下模型中提供6周的降解期。因此，通过交联和nZnHA的掺入来改善生物胶原膜的临床性能是一种很有前途的改善生物胶原膜的策略。意义声明本研究的意义包括：·我们通过GA-阿仑膦酸钠交联和nZnHA掺杂制备了一种具有增强的机械性能、延长的降解时间和不妥协的生物相容性的交联胶原膜。·调节锌离子的掺入浓度可以有效地控制所制备的膜的生物相容性和降解过程。我们提出了一个有前途的策略，以提高生物胶原蛋白膜的临床性能，通过交联结合nZnHA。
Biogenic collagen membranes have been widely used as soft tissue barriers in guided bone regeneration (GBR) and guided tissue regeneration (GTR). Nevertheless, their clinical performance remains unsatisfactory because of their low mechanical strength and fast degradation ratein vivo. Although cross-linking with chemical agents is effective and reliable for prolonging the degradation time of collagen membranes, some adverse effects including potential cytotoxicity and undesirable tissue integration have been observed during this process. As a fundamental nutritional trace element, zinc plays an active role in promoting the growth of cells and regulating the degradation of the collagen matrix. Herein, a biogenic collagen membrane was cross-linked with glutaraldehyde-alendronate to prolong its degradation time. The physiochemical and biological properties were enhanced by the incorporation of zinc-doped nanohydroxyapatite (nZnHA), with the native structure of collagen preserved. Specifically, the cross-linking combined with the incorporation of 1% and 2% nZnHA seemed to endow the membrane with the most appropriate biocompatibility and tissue integration capability among the cross-linked membranes, as well as offering a degradation period of six weeks in a rat subcutaneous model. Thus, improving the clinical performance of biogenic collagen membranes by cross-linking together with the incorporation of nZnHA is a promising strategy for the improvement of biogenic collagen membranes.Statement of significanceThe significance of this research includes:•We fabricated a cross-linked collagen membrane with enhanced mechanical properties, prolonged degradation time and uncompromised biocompatibility by GA-alendronate cross-linking and nZnHA doping.•Tuning the incorporation concentration of zinc ions can effectively manipulate the biocompatibility and degradation process of the membrane fabricated.•We proposed a promising strategy to improve the clinical performance of biogenic collagen membranes by cross-linking together with the incorporation of nZnHA.