Design of a Novel 3D Printed Bioactive Nanocomposite Scaffold for Improved Osteochondral Regeneration.
Design of a Novel 3D Printed Bioactive Nanocomposite Scaffold for Improved Osteochondral Regeneration.
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DOI：
10.1007/s12195-015-0389-4
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发表时间：
2015-09
影响因子：
2.8
通讯作者：
Zhang LG
中科院分区：
文献类型：
--
作者：
Castro NJ;Patel R;Zhang LG
由于组织固有的复杂性和较差的再生能力，骨关节炎和创伤导致的慢性和急性骨软骨缺损是一个常见而严重的临床问题。此外，骨软骨组织内的细胞与由许多生物活性有机和无机成分组成的三维纳米结构的细胞外基质密切接触。作为一种新兴的制造技术，3D打印对组织支架的微结构、形状和组成提供了很高的精度和控制。因此，本研究的目的是开发一种具有集成分化线索的仿生3D打印纳米复合支架，以改善骨软骨组织再生。通过有机和无机生物活性因子组成的新型纳米墨水与先进的3D打印技术的结合，我们成功地制造了一系列新颖的结构体，这些结构体具有纳米粒度、微观结构和时空生物活性线索，密切模仿了原生的3D细胞外环境。我们的研究结果说明了3D打印纳米复合材料支架的几个关键特征，包括改进的机械性能以及出色的细胞相容性，增强人骨髓源间充质干细胞的粘附、增殖和体外骨软骨分化。目前的工作进一步说明了这里开发的支架作为一个有前途的和高度可调的骨软骨组织再生平台的有效性。
Chronic and acute osteochondral defects as a result of osteoarthritis and trauma present a common and serious clinical problem due to the tissue's inherent complexity and poor regenerative capacity. In addition, cells within the osteochondral tissue are in intimate contact with a 3D nanostructured extracellular matrix composed of numerous bioactive organic and inorganic components. As an emerging manufacturing technique, 3D printing offers great precision and control over the microarchitecture, shape and composition of tissue scaffolds. Therefore, the objective of this study is to develop a biomimetic 3D printed nanocomposite scaffold with integrated differentiation cues for improved osteochondral tissue regeneration. Through the combination of novel nano-inks composed of organic and inorganic bioactive factors and advanced 3D printing, we have successfully fabricated a series of novel constructs which closely mimic the native 3D extracellular environment with hierarchical nanoroughness, microstructure and spatiotemporal bioactive cues. Our results illustrate several key characteristics of the 3D printed nanocomposite scaffold to include improved mechanical properties as well as excellent cytocompatibility for enhanced human bone marrow-derived mesenchymal stem cell adhesion, proliferation, and osteochondral differentiation in vitro. The present work further illustrates the effectiveness of the scaffolds developed here as a promising and highly tunable platform for osteochondral tissue regeneration.