POLYMERASE CHAIN-REACTION DETECTION OF PLASMODIUM-FALCIPARUM IN MOSQUITOS
POLYMERASE CHAIN-REACTION DETECTION OF PLASMODIUM-FALCIPARUM IN MOSQUITOS
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DOI：
10.1016/0035-9203(93)90124-9
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发表时间：
1993-05-01
影响因子：
2.2
通讯作者：
PANYIM, S
中科院分区：
文献类型：
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作者：
TASSANAKAJON, A;BOONSAENG, V;PANYIM, S
聚合酶链式反应 (PCR) 程序使用源自恶性疟原虫特异性重复脱氧核糖核酸 (DNA) 序列的引物组来检测蚊子中的寄生虫 DNA。在实验室感染的蚊子中，PCR 可以在解剖的唾液腺中检测到少至 10 个子孢子，在解剖的中肠中检测到单个卵囊。在野生捕获的蚊子中检测恶性疟原虫 DNA 的能力表明，在检测低度寄生虫感染的蚊子中的疟原虫子孢子方面，PCR 优于酶联免疫吸附测定。
The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections.