XRHAMM functions in Ran-dependent microtubule nucleation and pole formation during anastral spindle assembly
XRHAMM functions in Ran-dependent microtubule nucleation and pole formation during anastral spindle assembly
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DOI：
10.1016/j.cub.2004.10.002
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发表时间：
2004-10-26
期刊：
影响因子：
9.2
通讯作者：
Ohi, R
中科院分区：
文献类型：
--
作者：
Groen, AC;Cameron, LA;Ohi, R
背景：微管的有序组装对双极纺锤体的形成至关重要。根据细胞类型，微管通过两种不同的途径成核：中心体驱动或染色质驱动。染色质驱动的途径占主导地位的细胞缺乏centrosome.Results：人RHAMM（受体透明质酸介导的运动）最初涉及透明质酸诱导的运动，但已被证明与中心体，并发挥作用，在有丝分裂系统中的星形纺锤体极的完整性。我们已经确定了非洲爪蟾直系同源的人类RHAMM作为微管相关蛋白，在聚焦纺锤体极中起作用，并且在没有中心体的纺锤体组装过程中对于有效的微管成核至关重要。XRHAMM与γ-TuRC，一个复杂的微管成核所需的和TPX 2，微管成核和主轴极organization.Conclusions所需的蛋白质：XRHAMM促进RAN依赖，染色质驱动的成核过程中，可能需要协调激活TPX 2和γ-TuRC。
Background: The regulated assembly of microtubules is essential for bipolar spindle formation. Depending on cell type, microtubules nucleate through two different pathways: centrosome-driven or chromatin-driven. The chromatin-driven pathway dominates in cells lacking centrosomes.Results: Human RHAMM (receptor for hyaluronic-acid-mediated motility) was originally implicated in hyaluronic-acid-induced motility but has since been shown to associate with centrosomes and play a role in astral spindle pole integrity in mitotic systems. We have identified the Xenopus ortholog of human RHAMM as a microtubule-associated protein that plays a role in focusing spindle poles and is essential for efficient microtubule nucleation during spindle assembly without Centrosomes. XRHAMM associates both with gamma-TuRC, a complex required for microtubule nucleation and with TPX2, a protein required for microtubule nucleation and spindle pole organization.Conclusions: XRHAMM facilitates Ran-dependent, chromatin-driven nucleation in a process that may require coordinate activation of TPX2 and gamma-TuRC.