Effects of peripheral administration of LPS on the expression of immunoreactive interleukin-1 alpha, beta, and receptor antagonist in rat brain.
Effects of peripheral administration of LPS on the expression of immunoreactive interleukin-1 alpha, beta, and receptor antagonist in rat brain.
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LPS 外周给药对大鼠脑中免疫反应性白细胞介素 1 α、β 和受体拮抗剂表达的影响。
DOI：
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发表时间：
1998
影响因子：
5.2
通讯作者：
F. Tilders
中科院分区：
文献类型：
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作者：
A. van Dam;S. Poole;M. Schultzberg;F. Zavala;F. Tilders
细胞因子白细胞介素-1 （IL-1）似乎在感染期间脑介导的非特异性疾病症状的协调中起关键作用。在本研究中，我们研究了IL-1在中枢神经系统本身产生的可能性，它可能负责诱导脑介导的反应。利用免疫细胞化学技术，我们证明了细菌内毒素给药大鼠引起了时间（1.5-24小时）和剂量依赖性（4微克/千克-2.5毫克/千克）的IL - 1 β免疫反应性诱导，这些细胞被鉴定为脑膜和脉络膜丛中的巨噬细胞和脑各区域的小胶质细胞。在内毒素（2.5 mg/kg）作用8小时后，在与IL-1相同的区域和细胞类型中观察到免疫反应性IL-1 α。虽然没有进行定量测量，但似乎表达免疫反应性IL-1 α的细胞比表达IL-1 β的细胞少。此外，IL-1ra在室旁核和视上核的神经元中组成性表达，这与mRNA数据一致。给药后，我们观察到没有额外的细胞表达免疫反应性IL-1ra。我们得出的结论是，大脑中IL-1 α和IL-1 β的产生是在相同的细胞类型中诱导的，而IL-1ra是由不同的细胞类型组成的，可能是神经元。
The cytokine interleukin-1 (IL-1) appears to play a pivotal role in the orchestration of brain-mediated, nonspecific illness symptoms during an infection. In the present study, we examine the possibility that IL-1 is produced in the central nervous system itself, which may be responsible for the induction of brain-mediated responses. Using immunocytochemical techniques, we demonstrated that peripheral administration of bacterial endotoxin to rats caused a time- (1.5-24 hr) and dose-dependent (4 micrograms/kg-2.5 mg/kg) induction of IL 1 beta immunoreactivity in cells identified as macrophages in meninges and choroid plexus and microglial cells in various brain regions. At 8 hr after endotoxin (2.5 mg/kg), immunoreactive IL-1 alpha was observed in the same areas and cell types as IL-1 beta. Although no quantitative measurements have been performed, it appears that fewer cells express immunoreactive IL-1 alpha than IL-1 beta. Furthermore, IL-1ra was found to be constitutively expressed in neurons in the paraventricular nucleus and supraoptic nucleus, which is in accordance with mRNA data. After administration of endotoxin, we observed no additional cells that expressed immunoreactive IL-1ra. We conclude that IL-1 alpha and IL-1 beta production in the brain is induced in the same cell types, whereas IL-1ra is expressed constitutively by a different cell type--probably neurons.