Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag.
Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag.
复制标题
使用链霉亲和素结合肽（SBP）标签的ERK信号传导复合物的单步亲和纯化。
DOI：
10.1007/978-1-4939-6424-6_8
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Veraksa A
中科院分区：
文献类型：
--
作者：
Yang L;Veraksa A
Elucidation of biological functions of signaling proteins is facilitated by studying their protein–protein interaction networks. Affinity purification combined with mass spectrometry (AP-MS) has become a favorite method to study protein complexes. Here we describe a procedure for single-step purification of ERK (Rolled) and associated proteins from Drosophila cultured cells. The use of the streptavidin-binding peptide (SBP) tag allows for a highly efficient isolation of native ERK signaling complexes, which are suitable for subsequent analysis by mass spectrometry. Our analysis of the ERK interactome has identified both known and novel signaling components. This method can be easily adapted for SBP-based purification of protein complexes in any expression system.
