Cryo-electron microscopy for structure analyses of membrane proteins in the lipid bilayer
Cryo-electron microscopy for structure analyses of membrane proteins in the lipid bilayer
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DOI：
10.1016/j.sbi.2016.06.001
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发表时间：
2016-08-01
影响因子：
6.8
通讯作者：
Fujiyoshi, Yoshinori
中科院分区：
文献类型：
--
作者：
Abe, Kazuhiro;Fujiyoshi, Yoshinori
物质的不对称分布，如离子、溶质、化学物质和信息在生物膜上的分布，这些都是由膜蛋白产生的，是所有生物的重要标志。由于适当的不对称分布取决于膜蛋白在脂质双分子层中的精确功能，因此对这些蛋白的膜嵌入形式的结构和功能分析对于阐明其机制至关重要。在这篇综述中，我们提供了基于二维晶体电子晶体学的脂质双层膜蛋白结构分析的显著例子。我们还讨论了单粒子低温电子显微镜的最新发展，并提出了一种用于脂质双分子层膜蛋白结构分析的概念策略。
Asymmetric distributions of materials, such as ions, solutes, chemicals, and information across the bio-membrane, which are generated by membrane proteins, are crucial hallmarks of all living organisms. Since the appropriate asymmetric distribution depends on the precise function of membrane proteins in the lipid bilayer, structural and functional analyses of the membrane-embedded forms of these proteins are crucial toward elucidating their mechanisms.ln this review, we provide remarkable examples of structural analyses of membrane proteins in lipid bilayers based on electron crystallography of two-dimensional crystals. We also discuss the recent development of single-particle cryo-electron microscopy and propose a conceptual strategy for structural analysis of membrane proteins in the lipid bilayer.