PHOSPHORIBOSYL PYROPHOSPHATE AND THE MEASUREMENT OF INORGANIC PYROPHOSPHATE IN PLANT-TISSUES
PHOSPHORIBOSYL PYROPHOSPHATE AND THE MEASUREMENT OF INORGANIC PYROPHOSPHATE IN PLANT-TISSUES
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DOI：
10.1007/bf00392814
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发表时间：
1989-02-01
期刊：
影响因子：
4.3
通讯作者：
APREES, T
中科院分区：
文献类型：
--
作者：
DANCER, JE;APREES, T
这项工作提供了进一步的证据表明，植物含有可观量的无机焦磷酸盐（PPi），磷酸核糖焦磷酸盐（PPRibP）的分解并没有显着贡献的PPi在植物提取物中检测到。豌豆根提取物中的无机焦磷酸盐，斑叶天南星（Arum maculatum L.）和发育中的胚乳。用焦磷酸果糖6-磷酸1-磷酸转移酶（EC 2.7.1.90）和硫酸腺苷酰转移酶（EC 2.7.7.4）测定。两种不同的测定法给出了相同的PPi含量值和添加PPi的回收率值。结果表明，PPRibP在PPi的提取过程中转化为PPi。而A. maculatum和Z.与PPi的含量相比，玉米的含量可以忽略不计。
This work provides further evidence that plants contain appreciable amounts of inorganic pyrophosphate (PPi), and that breakdown of phosphoribosyl pyrophosphate (PPRibP) does not contribute significantly to the PPi detected in plant extracts. Inorganic pyrophosphate in extracts of the roots of Pisum sativum L., clubs of the spadices of Arum maculatum L., and the developing endosperm of Zea mays L. was assayed with pyrophosphate fructose 6-phosphate 1-phosphotransferase (EC 2.7.1.90), and with sulphate adenyltransferase (EC 2.7.7.4). The two different assays gave the same value for PPi content, and for recovery of added PPi. It was shown that PPRibP is converted to PPi during the extraction of PPi. However, the amounts of PPRibP in clubs of A. maculatum and the developing endosperm of Z. mays were negligible in comparison with the contents of PPi.