Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.
复制标题
泰国微小按蚊（双翅目：蚊科）A 种和 C 种的种内杂交。
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
D. Riyong
中科院分区：
文献类型：
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作者：
W. Choochote;Y. Rongsriyam;S. Leemingsawat;A. Jitpakdi;N. Komalamisra;K. Surathin;P. Somboon;Bin Chen;S. Wongkamchai;N. Jariyapan;P. Tippawangkosol;B. Pitasawat;D. Riyong
采用诱导交配的方法对小按蚊A种和C种的实验室饲养、等值线进行杂交试验。这三条等值线是根据野生捕获的完全丰满雌性的三种形态变异和两种不同类型的中期染色体建立的。他们是安。最小种A: V型（X1,Y1）， M型（X2,Y1）；C种：P型（X3,Y2）。反向杂交和回交结果表明，A种形态变异形式遗传亲和性好，可提供可存活的后代和完全突触的唾液腺多线染色体，而与C种和（或）P种形态不相容。杂种后代仅由两种形式的A种雌性x种C雄性获得，但在3L上的唾液腺多烯染色体和卵巢卵泡的部分发育中可以看到。F1杂交雄虫与亲本A种雌虫的回交产生了可存活的后代，而F1杂交雌虫与亲本C种雄虫的回交产生了低生存力的后代和精子异常的成年雄虫，说明了An的部分生殖隔离。最小种A和C。
Hybridization tests of laboratory-raised, isolines of Anopheles minimus, species A and C were conducted by induced copulation. The three isolines were established based on three morphological variants of wild-caught, fully engorged females and two distinct types of metaphase chromosomes. They were An. minimus species A: V form (X1,Y1), M form (X2,Y1); species C: P form (X3,Y2). The results of reciprocal and back crosses indicated that the two morphologically variant forms of species A were genetically compatible, providing viable progeny and completely synaptic salivary gland polytene chromosomes, whereas they were genetically incompatible with species C and/or the P form. Hybrid progeny was only obtained from both forms of species A females x species C males, but asynaptic salivary gland polytene chromosomes on 3L and partial development of ovarian follicles in females were seen. Back crosses of F1 hybrid males with parental species A females provided viable progeny, while back crosses of F1 hybrid females with parental species C males provided progeny of low viability and adult males with abnormal spermatozoa, suggesting the partial reproductive isolation of An. minimus species A and C.