DNA vaccine for hepatitis B: Evidence for immunogenicity in chimpanzees and comparison with other vaccines
DNA vaccine for hepatitis B: Evidence for immunogenicity in chimpanzees and comparison with other vaccines
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DOI：
10.1073/pnas.93.14.7213
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发表时间：
1996-07-09
影响因子：
11.1
通讯作者：
Purcell, RH
中科院分区：
文献类型：
--
作者：
Davis, HL;McCluskie, MJ;Purcell, RH
通过肌肉注射编码HBV主要和中间包膜蛋白的质粒DNA，对两只黑猩猩进行抗B肝炎病毒（HBV）疫苗接种，诱导组特异性抗体、亚型特异性抗体和preS 2特异性抗体。这些最初是IgM同种型，然后它们是IgG（主要是IgG 1）同种型。注射2 mg DNA的黑猩猩在一次注射后获得>100毫国际单位/ml的抗-HBs抗体，在四次注射后获得14，000毫国际单位/ml。较小的剂量（400 μ g）诱导较低和短暂的滴度，但强烈的回忆反应发生在1年后。在23只黑猩猩接受各种抗原为基础的HBV疫苗的反应比较表明，DNA的方法是有前途的预防性免疫接种HBV。
Vaccination of two chimpanzees against hepatitis B virus (HBV) by intramuscular injection of plasmid DNA encoding the major and middle HBV envelope proteins induced group-, subtype- and preS2-specific antibodies. These were initially of IgM isotype, and then they were of IgG (predominantly IgG1) isotype, The chimpanzee injected with 2 mg of DNA attained >100 milli-international units/ml of anti-HBs antibody after one injection and 14,000 milli-international units/ml after four injections. A smaller dose (400 mu g) induced lower and transient titers, but a strong anamnestic response occurred 1 year later. Comparison with responses in 23 chimpanzees receiving various antigen-based HBV vaccines suggests that the DNA approach is promising for prophylactic immunization against HBV.