Repeatable deep-tissue activation of persistent luminescent nanoparticles by soft X-ray for high sensitivity long-term in vivo bioimaging
Repeatable deep-tissue activation of persistent luminescent nanoparticles by soft X-ray for high sensitivity long-term in vivo bioimaging
复制标题
通过软 X 射线可重复激活持久发光纳米颗粒的深层组织，实现高灵敏度长期体内生物成像
DOI：
10.1039/c6nr09553d
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发表时间：
2017
期刊：
影响因子：
6.7
通讯作者：
Huanghao Yang
中科院分区：
文献类型：
--
作者：
Liang Song;Xiahui Lin;Xiaorong Song;Shan Chen;Xiaofeng Chen;Juan Li;Huanghao Yang
持久发光纳米颗粒（PLNPs）由于完全避免了组织自发荧光而成为生物成像的重要纳米材料。然而，成像灵敏度和长期的体内成像仍然是有限的，这是由于在离体激发后体内快速衰减的持续发光。为了解决这一限制，非常需要PLNP的体内活化。在此，我们提出了一种新的策略激活PLNPs（SrAl 2 O 4：Eu 2+）通过使用软X射线激发。重要的是，由于软X射线光源具有深入组织穿透的优点，PLNP可以使用软X射线激发通过活组织在体内重新激活。此外，可以实现X射线/持续发光双模成像，以使该策略具有高灵敏度。我们的研究结果表明，PLNP体内能量充电的新策略将为活体动物的深层组织和长期生物成像带来新的见解，并为持久发光生物成像和治疗应用提供新的前景。
Persistent luminescent nanoparticles (PLNPs) have emerged as important nanomaterials for biological imaging as a result of complete avoidance of tissue auto-fluorescence. However, the imaging sensitivity and long-term in vivo imaging are still limited due to the persistent luminescence that is rapidly decayed in vivo after an ex vivo excitation. To address this limitation, in vivo activation of PLNPs is highly desired. Herein, we present a new strategy for the activation of PLNPs (SrAl2O4:Eu2+) by using soft X-ray excitation. Importantly, as the soft X-ray light source possesses the advantage of deep tissue penetration, the PLNPs can be reactivated in vivo through living tissue using soft X-ray excitation. Furthermore, X-ray/persistent luminescence dual-modal imaging can be achieved to empower this strategy with high sensitivity. Our results suggest that this new strategy of in vivo energy charging in PLNPs would bring new insights for deep tissue and long-term bioimaging in living animals, and provide new perspectives for persistent luminescence bioimaging and therapeutic applications.