Transcript profiling in Candida albicans reveals new cellular functions for the transcriptional repressors CaTup1, CaMig1 and CaNrg1
Transcript profiling in Candida albicans reveals new cellular functions for the transcriptional repressors CaTup1, CaMig1 and CaNrg1
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DOI：
10.1046/j.1365-2958.2001.02713.x
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发表时间：
2001-11-01
影响因子：
3.6
通讯作者：
Brown, AJP
中科院分区：
文献类型：
--
作者：
Murad, AMA;d'Enfert, C;Brown, AJP
致病真菌白色念珠菌含有芽殖酵母中发现的转录抑制因子ScTup 1、ScMig 1和ScNrg 1的同源物。在酿酒酵母中，ScMig 1将ScTup 1/ScSsn 6复合物靶向葡萄糖抑制基因的启动子以抑制其转录。ScNrg 1被认为以类似的方式作用于其他启动子。我们研究了它们的同源物在C.白念珠菌的转录谱与阵列包含2002个基因，代表约四分之一的预测数量的开放阅读框（ORF）的C。白色念珠菌。数据显示，CaNrg 1和CaTup 1调节一组不同的C。白色念珠菌基因的CaMig 1和CaTup 1。这与CaMig 1和CaNrg 1将CaTup 1阻遏物靶向至C的特定亚群的想法一致。白色念珠菌基因。而CaMig 1和CaNrg 1则抑制其它C.白色念珠菌基因中的CaTup 1独立的方式。CaMig 1和CaNrg 1阻遏的靶点以及nrg 1/nrg 1和mig 1/mig 1突变体的表型分析表明，这些因子在代谢、细胞形态发生和应激反应的调节中发挥不同的作用。因此，这些数据提供了关于这些转录调节因子的作用模式及其细胞作用的重要信息。转录本分析数据可在http://www.pasteur.fr/recherche/ unites/RIF/transcriptdata/上获得。
The pathogenic fungus, Candida albicans contains homologues of the transcriptional repressors ScTup1, ScMig1 and ScNrg1 found in budding yeast. In Saccharomyces cerevisiae, ScMig1 targets the ScTup1/ScSsn6 complex to the promoters of glucose repressed genes to repress their transcription. ScNrg1 is thought to act in a similar manner at other promoters. We have examined the roles of their homologues in C. albicans by transcript profiling with an array containing 2002 genes, representing about one quarter of the predicted number of open reading frames (ORFs) in C. albicans. The data revealed that CaNrg1 and CaTup1 regulate a different set of C. albicans genes from CaMig1 and CaTup1. This is consistent with the idea that CaMig1 and CaNrg1 target the CaTup1 repressor to specific subsets of C. albicans genes. However, CaMig1 and CaNrg1 repress other C. albicans genes in a CaTup1-independent fashion. The targets of CaMig1 and CaNrg1 repression, and phenotypic analyses of nrg1/nrg1 and mig1/mig1 mutants, indicate that these factors play differential roles in the regulation of metabolism, cellular morphogenesis and stress responses. Hence, the data provide important information both about the modes of action of these transcriptional regulators and their cellular roles. The transcript profiling data are available at http://www.pasteur.fr/recherche/ unites/RIF/transcriptdata/.