RecA: Regulation and Mechanism of a Molecular Search Engine.
RecA: Regulation and Mechanism of a Molecular Search Engine.
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DOI：
10.1016/j.tibs.2016.04.002
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发表时间：
2016-06
影响因子：
13.8
通讯作者：
Kowalczykowski SC
中科院分区：
文献类型：
--
作者：
Bell JC;Kowalczykowski SC
同源重组通过修复断裂的染色体来维持基因组的完整性。断裂的染色体被部分切除以产生用于搜索同源dsDNA的单链DNA。这种同源性驱动的“搜索和救援”是由一类DNA链交换蛋白催化的，这些蛋白与大肠杆菌RecA有关，在单链DNA上形成细丝。在这里，我们回顾调节RecA丝组装和RecA快速，有效地搜索和识别一个独特的同源序列之间的大量过剩的异源DNA的机制。考虑到RecA是原型DNA链交换蛋白，其行为提供了对真核RAD 51直系同源物及其调节因子、BRCA 2和其他肿瘤抑制因子的作用的深入了解。
Homologous recombination maintains genomic integrity by repairing broken chromosomes. The broken chromosome is partially resected to produce single-stranded DNA that is used to search for homologous dsDNA. This homology-driven ‘search and rescue’ is catalyzed by a class of DNA strand exchange proteins that are defined in relation to Escherichia coli RecA, which forms a filament on single-stranded DNA. Here, we review the regulation of RecA filament assembly and the mechanism by which RecA quickly and efficiently searches for and identifies a unique homologous sequence amongst a vast excess of heterologous DNA. Given that RecA is the prototypic DNA strand exchange protein, its behavior affords insight into the actions of eukaryotic RAD51 orthologs and their regulators, BRCA2 and other tumor suppressors.