Transcription Factors WOX11/12 Directly Activate WOX5/7 to Promote Root Primordia Initiation and Organogenesis
Transcription Factors WOX11/12 Directly Activate WOX5/7 to Promote Root Primordia Initiation and Organogenesis
复制标题
转录因子WOX11/12直接激活WOX5/7促进根原基起始和器官发生
DOI：
10.1104/pp.16.01067
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发表时间：
2016-12-01
期刊：
影响因子：
7.4
通讯作者：
Xu, Lin
中科院分区：
文献类型：
--
作者：
Hu, Xiaomei;Xu, Lin
从头器官发生是植物再生的一种类型，它产生不定根和不定芽。在拟南芥（Arabidopsis thaliana）中，从叶外植体从头根器官发生过程中，需要两个主要的细胞命运转换步骤来建立根原基。从再生感受态细胞到根形成细胞的第一步涉及生长素激活WUSCHEL相关的HOMEOBOX 11（WOX 11）和WOX 12（WOX 11/12）表达。然而，控制第二步命运从根创始人细胞到根原基的分子机制知之甚少。在这项研究中，我们表明，WOX 11/12的表达水平下降，而WOX 5和7（WOX 5/7）的增加，从根创始人细胞的过渡到根原基。W 0X 11/12在W 0X 5/7的遗传上游起作用，并且W 0X 11/12蛋白直接结合W 0X 5/7的启动子以激活其转录。WOX 5/7突变导致原基形成缺陷。总的来说，我们的数据表明，表达开关从WOX 11/12到WOX 5/7是从头根器官发生过程中的根原基的启动至关重要。
De novo organogenesis, which gives rise to adventitious roots and shoots, is a type of plant regeneration for survival after wounding. In Arabidopsis (Arabidopsis thaliana), two main cell fate transition steps are required to establish the root primordium during de novo root organogenesis from leaf explants. The first step from regeneration-competent cells to root founder cells involves activation of WUSCHEL-RELATED HOMEOBOX11 (WOX11) and WOX12 (WOX11/12) expression by auxin. However, the molecular mechanism controlling the second step of fate transition from root founder cells to root primordium is poorly understood. In this study, we show that the expression levels of WOX11/12 decrease while those of WOX5 and 7 (WOX5/7) increase during the transition from root founder cells to the root primordium. WOX11/12 function genetically upstream of WOX5/7, and the WOX11/12 proteins directly bind to the promoters of WOX5/7 to activate their transcription. Mutations in WOX5/7 result in defective primordium formation. Overall, our data indicate that the expression switch from WOX11/12 to WOX5/7 is critical for initiation of the root primordium during de novo root organogenesis.