Interleukin-12 treatment during immunization elicits a T helper cell type 1-like immune response in mice challenged with respiratory syncytial virus and improves vaccine immunogenicity.
Interleukin-12 treatment during immunization elicits a T helper cell type 1-like immune response in mice challenged with respiratory syncytial virus and improves vaccine immunogenicity.
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免疫期间的白细胞介素 12 治疗可在呼吸道合胞病毒攻击的小鼠中引发类似 1 型 T 辅助细胞的免疫反应，并提高疫苗的免疫原性。
DOI：
10.1093/infdis/172.3.734
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Graham,BS
中科院分区：
文献类型：
--
作者：
Tang,YW;Graham,BS
本研究观察了外源性白细胞介素（IL）-12对呼吸道合胞病毒（RSV）感染小鼠免疫应答的辅助作用。在用福尔马林灭活的明矾沉淀的RSV制备物免疫时给予重组IL-12导致RSV攻击后4天肺中病毒复制的显著减少。当在免疫时给予腹膜内或肌内IL-12是有效的，但在攻击时不是。IL-12处理导致肺中干扰素-γ mRNA增加，IgG 2a RSV特异性抗体同种型利用增加，内源性IL-12 p40 mRNA表达增加。IL-12治疗对临床结果或细胞毒性T淋巴细胞活性无显著影响。这些数据表明，IL-12具有有效的佐剂作用，这可能是由于诱导T辅助细胞I型样免疫应答。
Adjuvant effects of exogenous interleukin (IL)-12 on induction of immune responses against respiratory syncytial virus (RSV) infection in mice were evaluated. Giving recombinant IL-12 at the time of immunization with a formalin-inactivated alum-precipitated RSV preparation resulted in significant reduction of virus replication in lungs 4 days after RSV challenge. Intraperitoneal or intramuscular IL-12 was effective when given at the time of immunization but not at the time of challenge. IL-12 treatment resulted in increased interferon-γ mRNA in lungs, increased IgG2a RSV-specific antibody isotype utilization, and increased endogenous IL-12 p40 mRNA expression. IL-12 treatment did not significantly affect clinical outcome or cytotoxic T lymphocyte activity. These data demonstrate that IL-12 has potent adjuvant effects that may be due to induction of T helper cell type I-like immune responses.