Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor.
Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor.
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DOI：
10.1093/nar/gkv1367
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发表时间：
2016-02-18
影响因子：
14.9
通讯作者：
Yarden Y
中科院分区：
文献类型：
--
作者：
Enuka Y;Lauriola M;Feldman ME;Sas-Chen A;Ulitsky I;Yarden Y
环状RNA（CircRNA）是广泛存在的功能未知的非编码RNA环。由于用表皮生长因子（EGF）刺激乳腺细胞导致编码和非编码RNA分子丰度的动态变化，并最终获得稳健的迁移表型，因此该细胞模型可能揭示circRNA的功能。在这里，我们发现EGF刺激的乳腺细胞的circRNA是稳定表达的，而mRNA和microRNA在几分钟内发生变化。一般来说，我们检测到的circRNA是相对长寿命和弱表达的。有趣的是，它们几乎普遍地与相应的线性转录物共表达，并且与产生线性同种型而没有可检测的circRNA的基因相比，各自的共享启动子区更有活性。这些发现意味着，与其他RNA水平的变化不同，circRNA丰度的改变可能在信号级联和下游转录网络中不起关键作用，这些网络迅速使细胞产生特定的结果。
Circular RNAs (circRNAs) are widespread circles of non-coding RNAs with largely unknown function. Because stimulation of mammary cells with the epidermal growth factor (EGF) leads to dynamic changes in the abundance of coding and non-coding RNA molecules, and culminates in the acquisition of a robust migratory phenotype, this cellular model might disclose functions of circRNAs. Here we show that circRNAs of EGF-stimulated mammary cells are stably expressed, while mRNAs and microRNAs change within minutes. In general, the circRNAs we detected are relatively long-lived and weakly expressed. Interestingly, they are almost ubiquitously co-expressed with the corresponding linear transcripts, and the respective, shared promoter regions are more active compared to genes producing linear isoforms with no detectable circRNAs. These findings imply that altered abundance of circRNAs, unlike changes in the levels of other RNAs, might not play critical roles in signaling cascades and downstream transcriptional networks that rapidly commit cells to specific outcomes.