X-ray observations of single bio-supramolecular photochirogenesis
X-ray observations of single bio-supramolecular photochirogenesis
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DOI：
10.1016/j.bpc.2018.07.003
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发表时间：
2018-11-01
影响因子：
3.8
通讯作者：
Sasaki, Yuji C.
中科院分区：
文献类型：
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作者：
Chang, Jaewon;Nishijima, Masaki;Sasaki, Yuji C.
2-蒽甲酸酯(AC)与人血清白蛋白(HSA)的结合行为和光生成行为迄今已被研究，并通过光谱分析和产物分析被理解为时间平均统计事件。在这项研究中，我们使用衍射X射线跟踪(DXT)技术来显示光照射下游离和AC负载的HSA(AC：HSA=0、1、5和10)以及AC-HSA络合物的单分子动力学，所有这些都被绑在金纳米晶体上，因此可以通过DXT实时跟踪。这使得我们能够在单分子分辨率下绘制更动态的生物超分子光发育的图景，详细描述了当ACS与其亚域间的IIA-IIB结合时，HSA的软化和灵活性增强，以及在光照射下产生的AC二聚体的动态挤出。
The binding and photochirogenic behaviour of 2-anthracenecarboxylate (AC) with human serum albumin (HSA) have hitherto been investigated and comprehended as time-averaged statistical events by spectroscopic examinations and product analyses. In this study, we employed a diffracted X-ray tracking (DXT) technique to visualize the single-molecular dynamics of free and AC-loaded HSA (AC:HSA = 0, 1, 5 and 10), as well as the AC-HSA complex under photoirradiation, all of which were tethered to gold nanocrystals and hence traceable in real time by DXT. This enabled us to draw a more dynamic picture of the bio-supramolecular photochirogenesis at a single-molecule resolution, detailing the softening and flexibility enhancement of HSA upon binding of ACs to its inter-subdomain IIA-IIB site and the dynamic extrusion of AC dimers produced upon photoirradiation.