To investigate protein evolution by detecting suppressed epitope structures.
To investigate protein evolution by detecting suppressed epitope structures.
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DOI：
10.1007/s00239-009-9202-0
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发表时间：
2009-05
影响因子：
3.9
通讯作者：
Jin, J. -P.
中科院分区：
文献类型：
--
作者：
Chong, Stephen M.;Jin, J. -P.
祖先核苷酸和蛋白质的物质残留在很大程度上是不可用的，因此现代生物中同源基因之间的序列比较形成了当前分子进化知识的核心。蛋白质三维结构的变异是功能多样性的基础。研究相关蛋白质三维结构的进化将大大提高我们对蛋白质进化和功能的理解。一个蛋白质可能含有祖先构象，这些构象被进化上的加性结构所抑制。我们的研究使用单克隆抗体探针在去除抑制子结构后检测蛋白质中的此类构象，证明了肌钙蛋白I和肌钙蛋白T（横纹肌Ca 2+调节系统中肌钙蛋白复合物的两个亚基）之间进化关系的三维结构证据。肌肉及其肌肉类型特异性亚型。实验数据表明，通过去除在进化过程中添加的构象调节剂片段来检测蛋白质中进化抑制的历史结构状态是可行的。除了确定对出现分歧蛋白质至关重要的结构修饰外，研究这种新的进化模式将有助于了解蛋白质结构的起源和功能潜力。
Material remains of ancestor nucleotides and proteins are largely unavailable, thus sequence comparison among homologous genes in present-day organisms forms the core of current knowledge of molecular evolution. Variation in protein three-dimensional structure is a basis for functional diversity. To study the evolution of three-dimensional structures in related proteins would significantly improve our understanding of protein evolution and function. A protein may contain ancestor conformations that have been allosterically suppressed by evolutionarily additive structures. Using monoclonal antibody probes to detect such conformation in proteins after removing the suppressor structure, our study demonstrated three-dimensional structure evidence for the evolutionary relationship between troponin I and troponin T, two subunits of the troponin complex in the Ca2+-regulatory system of striated muscle, and among their muscle type-specific isoforms. The experimental data showed the feasibility to detect evolutionarily suppressed history-telling structural states in proteins by removing conformational modulator segments added during evolution. In addition to identifying structural modifications that were critical to the emerging of diverged proteins, investigating this novel mode of evolution will help to understand the origin and functional potential of protein structures.
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影响因子：
3.5
作者：
Jin, JP;Chen, AH;Huang, QQ
通讯作者：
Huang, QQ
影响因子：
4.8
作者：
Jin, JP;Brotto, MA;Crawford, TO
通讯作者：
Crawford, TO
影响因子：
64.8
作者：
LI, WH;TANIMURA, M
通讯作者：
TANIMURA, M
DOI：
10.1152/ajpheart.91506.2007
发表时间：
2008-06-01
影响因子：
4.8
作者：
Du, Jianfeng;Liu, Jing;Huang, Xu-Pei
通讯作者：
Huang, Xu-Pei
影响因子：
4.8
作者：
Barbato, JC;Huang, QQ;Jin, JP
通讯作者：
Jin, JP