MONOCLONAL-ANTIBODIES AGAINST THE DROSOPHILA NERVOUS-SYSTEM
MONOCLONAL-ANTIBODIES AGAINST THE DROSOPHILA NERVOUS-SYSTEM
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DOI：
10.1073/pnas.79.24.7929
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
SHOTWELL, SL
中科院分区：
文献类型：
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作者：
FUJITA, SC;ZIPURSKY, SL;SHOTWELL, SL
用果蝇组织匀浆免疫小鼠，制备了148个针对果蝇神经抗原的单克隆抗体。抗体在蝇头低温切片上进行免疫组织化学筛选。观察到多种不同的染色模式。部分抗原在组织中广泛分布；另一些则高度特异于神经纤维、神经膜、肌肉、气管系统、细胞核、光感受器或其他结构。许多抗体的抗原在免疫印迹上被鉴定出来。识别神经系统内特定分子的单克隆抗体在神经发育的分子遗传学研究中应该证明是有用的。
A panel of 148 monoclonal antibodies directed against Drosophila neural antigens was prepared by using mice immunized with homogenates of Drosophila tissue. Antibodies were screened immunohistochemically on cryostat sections of fly heads. A large diversity of staining patterns was observed. Some antigens were broadly distributed among tissues; others were highly specific to nerve fibers, neuropil, muscle, the tracheal system, cell nuclei, photoreceptors or other structures. The antigens for many of the antibodies were identified on immunoblots. Monoclonal antibodies that identify specific molecules within the nervous system should prove useful in the study of the molecular genetics of neural development.