GENETIC-CONTROL OF WHISKER ANTIGEN OF BACTERIOPHAGE T4D
GENETIC-CONTROL OF WHISKER ANTIGEN OF BACTERIOPHAGE T4D
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DOI：
10.1016/0022-2836(74)90120-x
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
FRANKEL, FR
中科院分区：
文献类型：
--
作者：
DEWEY, MJ;WIBERG, JS;FRANKEL, FR
据报道，T4胡须（位于头尾连接处区域的短原纤维）的抗原成分受基因49的控制（Yanagida & Ahmad-Zadeh，1970; Yanagida，1972）。这是基于免疫学证据，使用了对吸附有突变体E727制备的基因49缺陷提取物的T4 D颗粒的抗血清。然而，后一种噬菌体在此显示为在基因49和另一个基因中携带琥珀突变的双突变体，所述另一个基因在本文中称为wac（须状抗原控制基因）。基因16的一般区域中的Genewacmaps。有证据表明胡须抗原是在wac而不是基因49的控制下。产生缺乏蛋白质的inwac缺陷型感染噬菌体。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析中，这种蛋白质似乎是抗原的主要成分。wac缺陷型噬菌体的尾纤维在野生型噬菌体的尾纤维沿尾折叠的条件下呈开放构型。因此，wac基因可能在尾纤维构型的调节中起作用。
An antigenic component of T4 whiskers (short fibrils located in the region of the head—tail junction) has been reported to be under the control of gene 49 (Yanagida & Ahmad-Zadeh, 1970; Yanagida, 1972). This was based on immunological evidence using antiserum to particles of T4D adsorbed with gene 49-defective extract made with the mutantamE727. The latter phage, however, is shown here to be a double mutant bearing amber mutations in gene 49 and another gene, herein referred to aswac(whiskerantigen control gene). Genewacmaps in the general region of gene 16. Evidence is presented indicating that the whisker antigen is under the control ofwacand not gene 49. Inwac-defective infections phage are produced that lack a protein. This protein appears by electrophoretic analysis in sodium dodecyl sulfate-polyacrylamide gels to be the major component of the antigen.The tail fibers ofwac-defective bacteriophage are in an open configuration under conditions in which those of wild-type phage are folded alongside the tail. Thus, thewacgene may have a role in the regulation of tail-fiber configuration.