Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta)
Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta)
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DOI：
10.1007/s10329-004-0084-x
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发表时间：
2004-07-01
期刊：
影响因子：
1.7
通讯作者：
Cabello, PH
中科院分区：
文献类型：
--
作者：
Andrade, MCR;Penedo, MCT;Cabello, PH
繁殖群体的长期管理需要对动物种群的遗传多样性进行一定程度的测量。为了维持用于生物医学研究的猴子的繁殖群体，已知的谱系提供精确的数据来确定群体的遗传状态。我们目前的遗传分析数据，在一个古老的封闭的恒河猴（猕猴），成立于1932年与100只动物的殖民地。40多年来，这些动物被关在一个孤岛上，1980年，建立了单一雄性繁殖群。共333个DNA样本，这些动物进行了分型，以20个微卫星标记，使用多重PCR，以验证近交系数（α）和杂合性水平。我们发现平均杂合性为64%，α = -0.03293（+/-0.00573）。我们的研究结果表明，使用的繁殖策略是有效的，因为近亲繁殖被避免。为了获得更高质量的灵长类动物用于生物医学研究，必须进行持续的遗传计划。
The long-term management of breeding colonies requires some measure of genetic diversity in the animal population. For the maintenance of breeding colonies of monkeys used for biomedical research, known pedigrees supply precise data to determine the genetic status of colonies. We present data of genetic analyses in an old closed colony of rhesus macaques (Macaca mulatta) that was established in 1932 with 100 animals. For more than 40 years, the animals were kept on an isolated island and, in 1980, single-male breeding groups were established. A total of 333 DNA samples of these animals were typed to 20 microsatellite markers using multiplex PCR in order to verify inbreeding coefficient (alpha) and level of heterozygosity. We found an average heterozygosity of 64% and obtained alpha = -0.03293 (+/- 0.00573). Our results indicate that the reproductive strategy used was effective because consanguineous breeding was avoided. A continuous genetic program must be carried out in order to obtain better quality primates for biomedical research.