Development of an Arbitrary Waveform Membrane Stretcher for Dynamic Cell Culture
Development of an Arbitrary Waveform Membrane Stretcher for Dynamic Cell Culture
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DOI：
10.1007/s10439-014-0976-x
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发表时间：
2014-05-01
影响因子：
3.8
通讯作者：
Black, Lauren D., III
中科院分区：
文献类型：
--
作者：
Lau, Jason J.;Wang, Raymond M.;Black, Lauren D., III
在本文中，一种新的细胞拉伸器的设计，模仿心脏壁的实时拉伸。通过在模拟天然心脏环境的机械方面的拉伸条件下培养细胞，可以更好地理解生物力学信号对细胞发育的作用。该设备利用移动磁体线性致动器，通过脉宽调制功率控制，结合自动闭环反馈系统，用于精确生成指定的机械拉伸轮廓。该系统的能力，以拉伸细胞培养膜和指定的频率和波形产生的准确性，循环拉伸进行了评估。温度和降解评估以及可扩展的设计证明了该系统在长期体外研究中的细胞培养应用。
In this paper, a novel cell stretcher design that mimics the real-time stretch of the heart wall is introduced. By culturing cells under stretched conditions that mimics the mechanical aspects of the native cardiac environment, better understanding on the role of biomechanical signaling on cell development can be achieved. The device utilizes a moving magnet linear actuator controlled through pulse-width modulated power combined with an automated closed loop feedback system for accurate generation of a designated mechanical stretch profile. The system's capability to stretch a cell culture membrane and accuracy of the designated frequency and waveform production for cyclic stretching were evaluated. Temperature and degradation assessments as well as a scalable design demonstrated the system's cell culture application for long term, in vitro studies.