Muscle fibers of mdx mice are more vulnerable to exercise than those of normal mice
Muscle fibers of mdx mice are more vulnerable to exercise than those of normal mice
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DOI：
10.1016/s0960-8966(97)00115-6
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发表时间：
1997-12-01
影响因子：
2.8
通讯作者：
Tremblay, JP
中科院分区：
文献类型：
--
作者：
Brussee, V;Tardif, F;Tremblay, JP
众所周知，离心运动通过破坏肌膜而引起肌肉损伤。本研究旨在分析下坡跑对正常及mdx小鼠几种运动及呼吸肌的影响。通过用伊文思蓝进行体内染色来观察小鼠骨骼肌中的变性肌纤维。静脉注射的这种染料仅染色变性的肌纤维，这些肌纤维在宏观上可见为蓝色纤维，在显微镜下也可见为红色荧光纤维。伊文思蓝染色的肌纤维要么过度收缩，要么变性。在没有运动的情况下，在正常小鼠中没有肌纤维被伊文思蓝标记，表明它们的膜是完整的。然而，即使没有运动，在mdx小鼠的各种肌肉中，伊文思蓝可渗透纤维的百分比也在2%至15%之间变化。我们的下坡跑步协议（即，以10 m/min的速度沿15度坡度的跑步机跑下）在正常小鼠中产生的染料可渗透的肌纤维的百分比仅略微增加（0-3%），而在一些MDX肌肉中可渗透的肌纤维的百分比高达31%。(C)1997年Elsevier Science B.V.
It is well known that eccentric exercise induces muscle damage by disrupting the sarcolemma. The aim of this study was to analyze the effects of downhill running on several locomotor and respiratory muscles of normal and mdx mice. Degenerating muscle fibers in the skeletal muscles of mice were visualized by in vivo staining with Evans blue. This dye injected intravenously stained only degenerating muscle fibers which were visible as blue fibers macroscopically and could also be seen as red fluorescent fibers microscopically. Evans blue-stained muscle fibers were either hypercontracted or degenerating. Without exercise no muscle fibers were labeled with Evans blue in the normal mice, indicating thai their membranes were intact. However, even without exercise, the percentage of fibers permeable to Evans blue varied from 2% to 15% in various muscles of the mdx mice. Our downhill running protocol (i.e., running down a treadmill with a 15 degrees slope at 10 m/min) produced in normal mice only a slight (0-3%) increase in percentage of muscle fibers which were permeable to the dye compared with up to 31% in some mdx muscles. (C) 1997 Elsevier Science B.V.