Development of the pelvic floor muscles of murine embryos with anorectal malformations
Development of the pelvic floor muscles of murine embryos with anorectal malformations
复制标题
DOI：
10.1053/jpsu.2002.30259
复制
发表时间：
2002-02-01
影响因子：
2.4
通讯作者：
Iwai, N
中科院分区：
文献类型：
--
作者：
Bitoh, Y;Shimotake, T;Iwai, N
背景/目的：近年来的生物学研究已经阐明了肌肉发育的分子机制，其中各种调控分子在胚胎发育过程中起着关键作用。为探讨肛门直肠畸形（anorectal malformations，ARM）中可能存在的肌源性异常，作者研究了ARM.Methods小鼠胚胎盆腔肌肉发育情况。从E10.5和E16.0之间的子宫获得胚胎标本，冷冻切片制备用于免疫组化，使用MyoD，myogenin和PGP9.5分子的特异性抗体。结果：在ARM胚胎中，神经管不规则分支，并在骶骨区域形成异常肿块。胚胎尾部体节分化成肌源性细胞，形成适当的肌管在骨盆对应的E12.5和E15.0之间的发育阶段，在受影响的胚胎和controls.Conclusions：在ARM胚胎，骨盆的解剖结构受损的神经发育不良，而肌肉发育生理进行。这些结果支持了这样的假设，即如果手术矫正得当，在排除了脊髓脊膜膨出或脊髓栓系等神经异常的ARM儿童中，盆底肌肉可能发挥作用。Copyright（C）2002 by W.B.桑德斯公司
Background/Purpose: Recent biological studies have elucidated the molecular mechanism of muscle development, in which various regulatory molecules play key roles during embryogenesis. To determine possible myogenic abnormalities in anorectal malformations (ARM), the authors investigated the pelvic muscle development in murine embryos affected with ARM.Methods: ARM embryos were induced by all-trans retinoic acid (ATRA) on the ninth gestational day (E9.0). Embryonal specimens were obtained from the uteri between E10.5 and E16.0, and the frozen sections were prepared for immunohistochemistry using antibodies specific for MyoD, myogenin, and PGP9.5 molecules.Results: In ARM embryos, the neural tube was irregularly branched and formed an anomalous mass in the sacral region. Embryonal caudal somites differentiated into myogenic cells to form proper myotubes in the pelvis corresponding to the developmental stages between E12.5 and E15.0 both in affected embryos and the controls.Conclusions: In ARM embryos, an impaired anatomic framework of the pelvis was caused by neural maldevelopment, whereas muscle development proceeded physiologically. These results support the hypothesis that pelvic floor muscles may function in ARM children, in whom neural abnormalities such as meningomyelocele or tethered spinal cord have been ruled out, if the surgical correction is appropriately completed. Copyright (C) 2002 by W.B. Saunders Company.