Potential differentiation of tumor bearing mouse CD11b+Gr-1+ immature myeloid cells into both suppressor macrophages and immunostimulatory dendritic cells
Potential differentiation of tumor bearing mouse CD11b+Gr-1+ immature myeloid cells into both suppressor macrophages and immunostimulatory dendritic cells
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DOI：
10.2220/biomedres.30.7
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发表时间：
2009-02-01
影响因子：
1.2
通讯作者：
Nishimura, Takashi
中科院分区：
文献类型：
--
作者：
Narita, Yoshinori;Wakita, Daiko;Nishimura, Takashi
Evaluation of immunosuppressive tumor-escape mechanisms in tumor-bearing hosts is of great importance for the development of ail efficient tumor immunotherapy. We document here the functional characteristics of CD11b(+)Gr-1(+) immature myeloid cells (ImC), which increase abnormally in tumor-bearing mice. Although it has been reported that ImC exhibit a strong immunosuppressive activity against T cell responses, we demonstrate that ImC derived from tumor-bearing mouse spleens (TB-SPL) did not exhibit a strong inhibitory activity against CTL generation in MLR. However, ImC isolated from TB-SPL and induced to differentiate into CD11b(+)Gr-1(+)F4/80(+) suppressor macrophages (M Phi) under the influence of tumor-derived factors were immunosuppressive. Furthermore, we also demonstrate that ImC isolated from TB-SPL had a capability of differentiating into immunostimulatory dendritic cells (DCl) supportive of the generation of IFN-gamma producing CTL if the ImC were cultured with Th1 cytokines Plus GM-CSF and IL-3. Thus, Our findings indicate that tumor bearing mouse-derived CD11b(+)Gr-1(+) ImC are not committed to development into immunosuppressor cells but have dual differentiation ability into both immunosuppressive myeloid cells and immunostimulatory DCl.
