Novel strategies for the biocontrol of noctuid pests (Lepidoptera) based on improving ascovirus infectivity using Bacillus thuringiensis.
Novel strategies for the biocontrol of noctuid pests (Lepidoptera) based on improving ascovirus infectivity using Bacillus thuringiensis.
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基于使用苏云金芽孢杆菌提高子囊病毒感染性的夜蛾类害虫（鳞翅目）生物防治新策略
DOI：
10.1111/1744-7917.12875
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发表时间：
2020
期刊：
影响因子：
4
通讯作者：
Huang Guo-Hua
中科院分区：
文献类型：
--
作者：
Yu Huan;Yang Chang-Jin;Li Ni;Zhao Ying;Chen Zhuang-Mei;Yi Si-Jia;Li Zi-Qi;Adang Michael J;Huang Guo-Hua
寻找新的生物防治剂和开发新的防治策略是害虫生物防治的迫切目标。子囊病毒是一种潜在的昆虫病毒，可能被开发成生物杀虫剂，但这一目标受到其较差的口腔传染性的阻碍。为了提高子囊病毒的感染性，采用苏云金芽孢杆菌（Bacillus thuringiensis kurstaki, Btk）作为辅助菌，对棉铃虫、分离密缕虫、狐尾蛾等鳞翅目幼虫的中肠进行破坏。HvAV - 3h和HvAV - 3j)的制剂。用Btk/HvAV‐3h和Btk/HvAV‐3j混合子囊病毒饲喂3龄昆虫幼虫。除了s。以食btk后死亡率较低的果蚜幼虫为例，其他被试幼虫对食配方有三种反应：ⅰ型，被试幼虫（棉铃虫）被btk感染后迅速死亡，没有足够的时间和资源供子囊病毒入侵；II型，两者的子囊病毒都被竞争耗尽，因此两者都不能成功释放或定植在组织中；III型btk被子囊病毒消灭，子囊病毒在受试幼虫中实现全身感染。饲喂btk /ascovirus配方饲料可显著降低幼虫的日食量，显著降低h。来,M。separata, and。实验结果表明，该配方对这些被试害虫的当代个体和下一代个体均有显著的口服控制效果。
Identifying novel biocontrol agents and developing new strategies are urgent goals in insect pest biocontrol. Ascoviruses are potential competent insect viruses that may be developed into bioinsecticides, but this aim is impeded by their poor oral infectivity. To improve theper osinfectivity of ascovirus,Bacillus thuringiensis kurstaki(Btk) was employed as a helper to damage the midgut of lepidopteran larvae (Helicoverpa armigera,Mythimna separata,Spodoptera frugiperda, andS. litura) in formulations withHeliothis virescens ascovirusisolates (HvAV‐3h and HvAV‐3j).Btkand ascovirus mixtures (Btk/HvAV‐3h andBtk/HvAV‐3j) were fed to insect larvae (3rd instar). With the exception ofS. frugiperdalarvae, which exhibited low mortality after ingestingBtk, the larvae of the other tested species showed three types of response to feeding on the formulas: type I, the tested larvae (H. armigera) were killed byBtkinfection so quickly that insufficient time and resources remained for ascoviral invasion; type II, bothBtkand the ascovirus were depleted by their competition, such that neither was successfully released or colonized the tissue; type III,Btkwas eliminated by the ascovirus, and the ascovirus achieved systemic infection in the tested larvae. The feeding ofBtk/ascovirus formulas led to a great reduction in larval diet consumption and resulted in a significant decrease in the emergence rate ofH. armigera,M. separata, andS. lituralarvae, which suggested that the formulas exerted marked oral control effects on both the contemporary individuals and the next generation of these tested pest species.