Octacalcium phosphate collagen composite stimulates the expression and activity of osteogenic factors to promote bone regeneration
Octacalcium phosphate collagen composite stimulates the expression and activity of osteogenic factors to promote bone regeneration
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DOI：
10.1002/term.2969
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发表时间：
2019-11-12
影响因子：
3.3
通讯作者：
Kamakura, Shinji
中科院分区：
文献类型：
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作者：
Kouketsu, Atsumu;Matsui, Keiko;Kamakura, Shinji
目的研究磷酸八钙胶原复合材料（OCP/Col）在大鼠颅骨骨缺损模型中的骨再生性能。设计将OCP/Col或β -磷酸三钙(β - tcp)/Col椎间盘植入临界尺寸的颅骨缺损，2或4周后固定。采用影像学检查和显微计算机断层扫描（mu-CT）检查椎间盘植入术后缺损的放射不透性。采用免疫组织化学和组织化学分析评估新生儿骨中骨基质的成熟、新生血管的形成以及破骨细胞和成骨细胞的分布。结果植入OCP/Col区域的x线和mu-CT检查显示新骨形成，与原骨无差异。采用免疫组化和组织化学方法检测新生颅骨骨及周围结缔组织中骨桥蛋白、骨钙素、runt相关转录因子2、1型胶原、血管内皮生长因子、碱性磷酸酶或抗酒石酸酸性磷酸酶的表达。缺陷部位的生物标志物表达未显著升高；其面积是通过将健康骨缘或颅骨与硬脑膜表面的距离除以来计算的。在术后2周和4周，OCP/Col组中这些生物标志物的表达没有差异。此外，术后2周和4周，OCP/Col组所有标志物的表达水平均高于β - tcp /Col组。结论OCP/Col作为骨再生材料，不仅表现出依赖于残余健康骨组织的骨传导活性，而且具有诱导成骨的能力，促进宿主组织血管生成和成骨细胞向骨缺损的侵袭。
Objective This study investigated the bone regenerative properties of an octacalcium phosphate collagen composite (OCP/Col) in a rat calvarial bone defect model. Design An OCP/Col or beta-tricalcium phosphate (beta-TCP)/Col disk was implanted into the critical-sized calvarial defects and fixed 2 or 4 weeks later. The radiopacity of defects was examined after disk implantation by the radiographic examination and micro-computed tomography (mu-CT). Immunohistochemical and histochemical analyses were carried out to assess the bone matrix maturation, neovascularization, and osteoclast and osteoblast distribution in the neonatal bone. Results Radiographic and mu-CT examination of the area of implanted OCP/Col indicated the newly formed bone and no difference from those of the original bone. Osteopontin, osteocalcin, Runt-related transcription factor 2, type 1 collagen, vascular endothelial growth factor, and alkaline phosphatase or tartrate-resistant acid phosphatase in the newly formed calvarial bone and the surrounding connective tissue were detected by immunohistochemistry and histochemistry. Biomarker expression was not significantly elevated at the defect site; the area of which was calculated by dividing the distance from the healthy bone margin or calvarium and dura mater surface. There was no difference in the expression of these biomarkers in the OCP/Col group at 2 and 4 weeks after surgery. In addition, the expression levels of all markers were higher in the OCP/Col group than in the beta-TCP/Col group at 2 and 4 weeks after surgery. Conclusions The OCP/Col as a bone regeneration material not only exhibits osteoconductive activity that is dependent on residual healthy bone tissue, but also has osteoinductive capacity, which promotes angiogenesis and osteogenic cell invasion from host tissue into the bone defect.