Identification and activity of a bacterial strain for the biodegradation of microcystins.
Identification and activity of a bacterial strain for the biodegradation of microcystins.
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发表时间：
2011
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通讯作者：
Li Yu-cheng
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作者：
Li Yu-cheng
对采自巢湖的一株微囊藻毒素降解菌M9进行了生理生化特性和16S rDNA序列的同源性分析。BLAST序列分析表明，该菌株与吉氏枯草杆菌有99%的相似性。考察了pH值、外加碳源、氮源和金属离子对M9菌株降解微囊藻毒素的影响。M9菌株降解微囊藻毒素的最适pH值为7.0。与葡萄糖和甘油相比，乙醇是菌株M9降解微囊藻毒素的较好碳源。微囊藻毒素-RR(MC-RR)和微囊藻毒素-LR(MC-LR)在48h内的降解率分别为70.0%和81.4%。与其他氮源相比，(NH4)2SO4是M9菌株的最佳氮源，MC-RR和MC-LR在48h的降解率分别为70.4%和80.9%。结果表明，Cu2+、Zn2+和Mn2+均能促进MCs的生物降解率，48h内MC-RR和MC-LR的降解率分别高于85%和93%。Fe~(3+)、Mg~(2+)对MC的生物降解没有明显的促进作用。
A microcystin-degrading bacterial strain M9 isolated from Chaohu Lake was identified by physiological-biochemical characteristics and homology analysis of 16S rDNA gene sequence.BLAST sequence analysis showed that it had a 99% similarity to kurthia gibsonii.The effects of pH,additional carbon and nitrogen sources,and metal ions on the biodegradation activity of microcystins(MCs) by strain M9 were investigated.pH7.0 was the best value for the biodegradation of MCs by strain M9.Compared with glucose and glycerol,ethanol was found to be a better carbon source for the biodegradation of MCs by strain M9,and the biodegradation rates of microcystin-RR(MC-RR) and microcystin-LR(MC-LR) in 48h were 70.0% and 81.4%,respectively.(NH4)2SO4 was the best nitrogen source for strain M9 comparing with other nitrogen sources,and the biodegradation rates of MC-RR and MC-LR in 48h were 70.4% and 80.9%,respectively.It was shown that the biodegradation of MCs could be increased with Cu2+,Zn2+ and Mn2+,and the biodegradation rates of MC-RR and MC-LR in 48h were higher than 85% and 93%,respectively.However,obvious acceleration with Fe3+ and Mg2+ for the biodegradation of MCs was not observed.