The switch-on luminescence sensing of histidine-rich proteins in solution: a further application of a Cu2+ ligand.
The switch-on luminescence sensing of histidine-rich proteins in solution: a further application of a Cu2+ ligand.
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DOI：
10.1039/c1ob05372h
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发表时间：
2011-05
影响因子：
3.2
通讯作者：
Bin Wang;Yang Gao;Hongwei Li;Zhi-peng Hu;Yuqing Wu
中科院分区：
文献类型：
--
作者：
Bin Wang;Yang Gao;Hongwei Li;Zhi-peng Hu;Yuqing Wu
在改进的Dansyl-Gly-Py（1）探针的基础上，通过模拟肽ATCUN的结构，研制了一种新的探针/Cu（2+）配合物，用于检测组氨酸标记的蛋白质。在水溶液中，1/Cu（2+）对组氨酸和半胱氨酸有很好的选择性，并可通过释放1的猝灭荧光来检测富组氨酸蛋白。
A new probe/Cu(2+) complex for the detection of his-tagged protein has been developed, based on an improved probe, Dansyl-Gly-Py (1), by closely mimicing the structure of a peptide, ATCUN. In aqueous solution, 1/Cu(2+) has good selectivity to histidine and cysteine, and further can detect histidine-rich protein by releasing the quenched fluorescence of 1.