8-Prenylnaringenin promotes recovery from immobilization-induced disuse muscle atrophy through activation of the Akt phosphorylation pathway in mice
8-Prenylnaringenin promotes recovery from immobilization-induced disuse muscle atrophy through activation of the Akt phosphorylation pathway in mice
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8-异戊二烯柚皮素通过激活小鼠的 Akt 磷酸化途径促进固定诱导的废用性肌肉萎缩的恢复
DOI：
10.1152/ajpregu.00521.2015
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Terao J*
中科院分区：
文献类型：
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作者：
Mukai R;Horikawa H;Lin PY;Tsukumo N;Nikawa T;Kawamura T;Nemoto H;Terao J*
8-丙基柚皮苷(8-PN)是一种从啤酒花提取物中提取的戊烯基黄酮类化合物，被认为有助于防止废用肌肉萎缩。我们推测，8-PN通过促进废用性肌肉萎缩后的肌肉恢复来影响肌肉的可塑性。为了测试8-PN对肌肉恢复的促进作用，我们用8-PN混合饲料给一条腿石膏固定了14天的小鼠。摄入8-PN混合饮食加速了肌肉萎缩的恢复，并防止了Akt磷酸化的减少。对体外培养的小鼠肌管细胞的研究表明，生理浓度的8-PN可激活PI3K/Akt/P70S6K1通路。使用雌激素受体抑制剂的细胞培养研究和去卵巢小鼠的体内实验表明，8-PN的雌激素活性有助于通过激活Akt磷酸化途径从废用性肌肉萎缩中恢复过来。这些数据强烈表明，8-PN是一种天然存在的化合物，可以用作营养补充剂，帮助从废用肌肉萎缩中恢复过来。
8-Prenylnaringenin (8-PN) is a prenylflavonoid that originates from hop extracts and is thought to help prevent disuse muscle atrophy. We hypothesized that 8-PN affects muscle plasticity by promoting muscle recovery under disuse muscle atrophy. To test the promoting effect of 8-PN on muscle recovery, we administered an 8-PN mixed diet to mice that had been immobilized with a cast to one leg for 14 days. Intake of the 8-PN mixed diet accelerated recovery from muscle atrophy, and prevented reductions in Akt phosphorylation. Studies on cell cultures of mouse myotubes in vitro demonstrated that 8-PN activated the PI3K/Akt/P70S6K1 pathway at physiological concentrations. A cell-culture study using an inhibitor of estrogen receptors and an in vivo experiment with ovariectomized mice suggested that the estrogenic activity of 8-PN contributed to recovery from disuse muscle atrophy through activation of an Akt phosphorylation pathway. These data strongly suggest that 8-PN is a naturally occurring compound that could be used as a nutritional supplement to aid recovery from disuse muscle atrophy.