Skeletal myogenesis on highly orientated microfibrous polyesterurethane scaffolds
Skeletal myogenesis on highly orientated microfibrous polyesterurethane scaffolds
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DOI：
10.1002/jbm.a.31534
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发表时间：
2008-03-15
影响因子：
4.9
通讯作者：
Mantero, S.
中科院分区：
文献类型：
--
作者：
Riboldi, S. A.;Sadr, N.;Mantero, S.
骨骼肌发生是一个复杂的过程，众所周知，它密切依赖于一个最佳的外向内底物细胞信号。目前使用传统支架在体外复制骨骼肌组织的尝试主要是由于肌纤维的方向性差，导致不能有效地产生矢量能量。在这项研究中，我们旨在研究由DegraPol(R)制成的新型可生物降解微纤维支架上的骨骼肌发生，DegraPol(R)是一种先前证明适合这种应用的嵌段聚酯酯。DegraPol(R)通过静电纺丝以高度取向(“O”)和无取向(“N/O”)微纤维网的形式进行加工，并通过溶剂浇铸以无孔薄膜(“F”)的形式进行。通过观察C2C12和L6的增殖(通过扫描电子显微镜分析和AlamarBlue(TM)试验)和分化(通过RT-PCR分析和MHC免疫染色)来评价纤维在支架表面取向的影响。我们证明，高度定向的弹性微纤维DegraPol(R)支架通过提供必要的方向线索以及建筑和机械支持，帮助(A)成肌细胞黏附、(B)肌管排列和(C)细胞的非共面排列，从而使骨骼肌在体外发生。(C)2007年威利期刊公司。
Skeletal myogenesis is a complex process, which is known to be intimately depending on an optimal outside-in substrate-cell signaling. Current attempts to reproduce skeletal muscle tissue in vitro using traditional scaffolds mainly suffer from poor directionality of the myofibers, resulting in an ineffective vectorial power generation. In this study, we aimed at investigating skeletal myogenesis on novel biodegradable microfibrous scaffolds made of DegraPol (R), a block polyesterurethane previously demonstrated to be suitable for this application. DegraPol (R) was processed by electrospinning in the form of highly orientated ("O") and nonorientated ("N/ O") microfibrous meshes and by solvent-casting in the form of nonporous films ("F"). The effect of the fiber orientation at the scaffold surface was evaluated by investigating C2C12 and L6 proliferation (via SEM analysis and alamarBlue (TM) test) and differentiation (via RT-PCR analysis and MHC immunostaining). We demonstrated that highly orientated elastomeric microfibrous DegraPol (R) scaffolds enable skeletal myogenesis in vitro by aiding in (a) myoblast adhesion, (b) myotube alignment, and (c) noncoplanar arrangement of cells, by providing the necessary directional cues along with architectural and mechanical support. (C) 2007 Wiley Periodicals, Inc.