Serologic and molecular comparisons of several equine herpesvirus type 1 strains.
Serologic and molecular comparisons of several equine herpesvirus type 1 strains.
复制标题
几种马疱疹病毒 1 型毒株的血清学和分子比较。
DOI：
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发表时间：
1981
影响因子：
1
通讯作者：
J. Bryans
中科院分区：
文献类型：
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作者：
L. Turtinen;G. Allen;R. Darlington;J. Bryans
将最近分离的2株马疱疹病毒1型呼吸道分离株（命名为T1和T2）与Army 183、M-A仓鼠适应株（KyA-ha）和L-M细胞适应株（KyA-LM）的分子和血清学相关性进行了比较。聚丙烯酰胺凝胶电泳结果显示，4株纯化菌株的病毒粒子结构蛋白存在差异。七种包膜糖蛋白（分子量为93，000、65，000、62，000、60，000、36，000、20，000和18，000），分别对应于病毒体蛋白13、16、17、18、23、25和26 a，在Army 183和KyA-ha菌株中发现的T1和T2分离株中的对应物具有略微不同的分子量，其具有相同的结构蛋白谱。病毒体蛋白19（58，000道尔顿），一种非糖基化蛋白，在呼吸道分离物中以减少的量存在，而病毒体蛋白8A（200，000道尔顿）不存在。病毒粒子蛋白8a是一种包膜糖蛋白，仅存在于KyA-ha株中。T_1和T_2分离株不能被马疱疹病毒2型抗血清中和，与Army 183和KyA-ha株在空斑减少中和试验中几乎没有交叉中和作用。病毒DNA的限制性内切酶切割图谱显示T1和T2分离株之间DNA片段的数量和大小相似，但不相同。同样，Army 183、KyA-ha和KyA-LM的DNA谱也彼此相似，但与呼吸道分离株有很大不同。
The molecular and serologic relatedness of 2 recent respiratory tract isolates of equine herpesvirus type 1, designated T1 and T2, were compared with the Army 183, Kentucky-A hamster-adapted (KyA-ha), and L-M cell-adapted (KyA-LM) strains. Electrophoresis in polyacrylamide gels revealed differences in virion structural proteins among 4 purified strains. Seven envelope glycoproteins (molecular weight of 93,000, 65,000, 62,000, 60,000, 36,000, 20,000, and 18,000) corresponding to virion proteins 13, 16, 17, 18, 23, 25, and 26a, respectively, found in both the Army 183 and KyA-ha strains had slightly different molecular weight counterparts in both the T1 and T2 isolates, which had identical structural protein profiles. virion protein 19 (58,000 daltons), a nonglycosylated protein, was present in reduced amounts in the respiratory tract isolates, whereas virion protein 8a (200,000 daltons) was absent. Virion protein 8a, an envelope glycoprotein, was only present in the KyA-ha strain. The T1 and T2 isolates were not neutralized by equine herpesvirus type 2 antiserum and revealed little cross-neutralizatio with the Army 183 and KyA-ha strains in plaque-reduction neutralization tests. Restriction endonuclease cleavage maps of viral DNA revealed a similar, but not identical, number and size of DNA fragments between T1 and T2 isolates. Likewise, DNa profiles of Army 183, KyA-ha, and KyA-LM were also similar to each other, but vastly different from the respiratory tract isolates.