Gene transfer to plants by diverse species of bacteria
Gene transfer to plants by diverse species of bacteria
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DOI：
10.1038/nature03309
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发表时间：
2005-02-10
期刊：
影响因子：
64.8
通讯作者：
Jefferson, RA
中科院分区：
文献类型：
--
作者：
Broothaerts, W;Mitchell, HJ;Jefferson, RA
农杆菌被广泛认为是唯一能够将基因转移到植物中的细菌属。经过适当修饰后，农杆菌已成为植物生物技术中最有效的基因转移载体(1)。然而，专利格局的复杂性(2) 为全球许多公共和私人组织有效利用该技术进行农业改良造成了现实和感知上的障碍。在这里，我们展示了农杆菌属以外的几种细菌可以被修饰以介导基因转移到许多不同的植物中。通过获得解除武装的Ti质粒和合适的双元载体，使这些与植物相关的共生细菌具有基因转移能力。这种用于作物改良的农杆菌介导技术的替代方案，除了为植物生物技术提供多功能“开源”平台之外，还可能导致天然细菌-植物相互作用的新用途，以实现植物转化。
Agrobacterium is widely considered to be the only bacterial genus capable of transferring genes to plants. When suitably modified, Agrobacterium has become the most effective vector for gene transfer in plant biotechnology(1). However, the complexity of the patent landscape(2) has created both real and perceived obstacles to the effective use of this technology for agricultural improvements by many public and private organizations worldwide. Here we show that several species of bacteria outside the Agrobacterium genus can be modified to mediate gene transfer to a number of diverse plants. These plant-associated symbiotic bacteria were made competent for gene transfer by acquisition of both a disarmed Ti plasmid and a suitable binary vector. This alternative to Agrobacterium-mediated technology for crop improvement, in addition to affording a versatile 'open source' platform for plant biotechnology, may lead to new uses of natural bacteria-plant interactions to achieve plant transformation.