Bone morphogenetic protein- and mating-dependent secretory cell growth and migration in the Drosophila accessory gland
Bone morphogenetic protein- and mating-dependent secretory cell growth and migration in the Drosophila accessory gland
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DOI：
10.1073/pnas.1214517109
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发表时间：
2012-11-20
影响因子：
11.1
通讯作者：
Wilson, Clive
中科院分区：
文献类型：
--
作者：
Leiblich, Aaron;Marsden, Luke;Wilson, Clive
黑腹果蝇的成对雄性副腺通过将分泌上皮的复杂糖蛋白混合物释放到精液中，增强精子功能，刺激卵子产生，并降低雌性对其他雄性的接受能力。每个腺体的远端都有一个由大约 40 个专门分泌细胞组成的小亚群，称为次级细胞。我们证明这些细胞通过交配促进的机制生长。如果衰老的雄性反复交配，这些细胞的一部分会从腺上皮的顶端表面分层并沿着腺体的近端迁移。值得注意的是，这些分泌细胞可以在交配过程中将精子转移给雌性。这一事件的频率随着年龄的增长而增加，因此超过 50% 的三交、18 岁的雄性将次级细胞转移给雌性。需要专门在次生细胞中的骨形态发生蛋白信号传导来驱动所有这些过程，并且需要辅助腺体对多次交配的雄性中的雌性交配后行为产生正常影响。我们得出的结论是，次级细胞是具有不寻常的迁移特性的分泌细胞，可以使它们转移到雌性身上，并且这些特性是随着雄性衰老和交配，次级细胞维持其正常生殖功能所需的信号传导的结果。
The paired male accessory glands of Drosophila melanogaster enhance sperm function, stimulate egg production, and reduce female receptivity to other males by releasing a complex mixture of glycoproteins from a secretory epithelium into seminal fluid. A small subpopulation of about 40 specialized secretory cells, called secondary cells, resides at the distal tip of each gland. We show that these cells grow via mechanisms promoted by mating. If aging males mate repeatedly, a subset of these cells delaminates from and migrates along the apical surface of the glandular epithelium toward the proximal end of the gland. Remarkably, these secretory cells can transfer to females with sperm during mating. The frequency of this event increases with age, so that more than 50% of triple-mated, 18-d-old males transfer secondary cells to females. Bone morphogenetic protein signaling specifically in secondary cells is needed to drive all of these processes and is required for the accessory gland to produce its normal effects on female postmating behavior in multiply mated males. We conclude that secondary cells are secretory cells with unusual migratory properties that can allow them to be transferred to females, and that these properties are a consequence of signaling that is required for secondary cells to maintain their normal reproductive functions as males age and mate.