Identification of QTLs for heat tolerance at the booting stage in rice （Oryza saliva L.）
Identification of QTLs for heat tolerance at the booting stage in rice （Oryza saliva L.）
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发表时间：
2006
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通讯作者：
Zhigang Zhao;Ling Jiang;Yinghui Xiao;Wenwei Zhang;H. Zhai;J. Wan
中科院分区：
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作者：
Zhigang Zhao;Ling Jiang;Yinghui Xiao;Wenwei Zhang;H. Zhai;J. Wan
利用水稻的杂交优势是提高水稻产量的重要途径。然而，由于温度异常对小穗不育的影响而导致的产量下降一直限制着水稻杂交水稻的应用。本研究的目的是寻找与高温下水稻杂交水稻颖花育性相关的数量性状基因座(QTL)。构建了苏糖5号/广节9号/苏苏5号回交群体及其分子图谱。用小穗高温育性和小穗育性热敏指数评价水稻孕穗期对高温胁迫的耐性。这些与孕穗期耐热性相关的QTL分别位于第2、4和5号染色体上，可解释6.4%~15.8%的表型变异。检测到2个与孕穗期相对耐热性相关的QTL，位于第4和第8染色体上的LOD分别为3.81和2.86，可解释表型变异的16.8%和9.9%。对相对耐热性的上位性QTL进一步分析表明，4对座位在8条染色体上存在互作，这表明颖花育性的耐热性不仅受主效QTL控制，而且还受基因互作的影响。
The utilization of heterosis between indica and japonica rice is an important approach to improve the productivity of rice. However, the reduced productivity due to the influence of abnormal temperature on spikelet sterility has been limiting the application of the indica-japonica cross of rice. The objective of this study was to identify the quantitative traits loci （QTLs） related to the spikelet fertility of the indica-japonica cross of rice under high growth temperature. The backcross population of USSR_(5)/Guangjie 9//USSR_(5) and related molecular map were constructed. The spikelet fertility under high temperature and heat susceptibility index （HSI） of spikelet fertility were used to evaluate the tolerance of rice to heat stress at booting stage, respectively. These QTLs related to heat tolerance at the booting stage were detected on chromosomes 2, 4 and 5, respectively, which explained 6.4%-15.8% of the observed phenotypic variance. Two QTLs related to relative heat tolerance at the booting stage were detected, with LOD scoring 3.81 and 2.86 on chromosome 4 and 8, and explained 16.8% and 9.9% of the phenotypic variance, respectively. Further analysis of epistatic effected QTLs for relative heat tolerance showed that four pairs of loci exhibited interaction on 8 chromosomes, which indicated that the heat tolerance of spikelet fertility was not only controlled by main effect QTL but also influenced by gene interaction.