Yeast 9-1-1 complex acts as a sliding clamp for DNA synthesis by DNA polymerase ε.
Yeast 9-1-1 complex acts as a sliding clamp for DNA synthesis by DNA polymerase ε.
复制标题
酵母9-1-1复合物充当DNA聚合酶ε的DNA合成的滑动夹。
DOI：
10.1016/j.jbc.2022.102727
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发表时间：
2023-01
期刊：
影响因子：
--
通讯作者：
Prakash S
中科院分区：
文献类型：
--
作者：
Acharya N;Prakash L;Prakash S
Eukaryotic cells harbor two DNA-binding clamps, proliferating cell nuclear antigen (PCNA), and another clamp commonly referred to as 9-1-1 clamp. In contrast to the essential role of PCNA in DNA replication as a sliding clamp for DNA polymerase (Pol) δ, no such role in DNA synthesis has been identified for the human 9-1-1 clamp or the orthologous yeast 17-3-1 clamp. The only role identified for either the 9-1-1 or 17-3-1 clamp is in the recruitment of signal transduction kinases, which affect the activation of cell cycle checkpoints in response to DNA damage. However, unlike the loading of PCNA by the replication factor C (RFC) clamp loader onto 3′-recessed DNA junctions for processive DNA synthesis by Polδ, the 17-3-1 clamp or the 9-1-1 clamp is loaded by their respective clamp loader Rad24-RFC or RAD17-RFC onto the 5′-recessed DNA junction of replication protein A–coated DNA for the recruitment of signal transduction kinases. Here, we identify a novel role of 17-3-1 clamp as a sliding clamp for DNA synthesis by Polε. We provide evidence that similar to the loading of PCNA by RFC, the 17-3-1 clamp is loaded by the Rad24-RFC clamp loader at the 3′-recessed DNA junction in an ATP-dependent manner. However, unlike PCNA, the 17-3-1 clamp does not enhance the processivity of DNA synthesis by Polε; instead, it greatly increases the catalytic efficiency of Polε for correct nucleotide incorporation. Furthermore, we show that the same PCNA-interacting peptide domain in the polymerase 2 catalytic subunit mediates Polε interaction with the 17-3-1 clamp and with PCNA.
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影响因子：
4.8
作者：
Ellison, V;Stillman, B
通讯作者：
Stillman, B
影响因子：
5.6
作者：
Sohn, Sun Young;Cho, Yunje
通讯作者：
Cho, Yunje
影响因子：
3.8
作者：
Sukhanova, Maria V.;D'Herin, Claudine;Lavrik, Olga I.
通讯作者：
Lavrik, Olga I.
影响因子：
4.8
作者：
Lee, Joon;Kumagai, Akiko;Dunphy, William G.
通讯作者：
Dunphy, William G.
DOI：
10.1073/pnas.0437927100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Bermudez, VP;Lindsey-Boltz, LA;Sancar, A
通讯作者：
Sancar, A