Chronic inflammatory responses to microgel-based implant coatings
Chronic inflammatory responses to microgel-based implant coatings
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DOI：
10.1002/jbm.a.32669
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发表时间：
2010-07-01
影响因子：
4.9
通讯作者：
Garcia, Andres J.
中科院分区：
文献类型：
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作者：
Bridges, Amanda W.;Whitmire, Rachel E.;Garcia, Andres J.
对植入生物医学设备的炎症反应会引起异物纤维化反应，限制设备在各种生物医学应用中的集成和性能。我们研究了微凝胶保形涂层的慢性炎症反应，该涂层由沉积在聚对苯二甲酸乙二醇酯（PET）上的聚（N-异丙基丙烯酰胺）水凝胶微粒与聚（乙二醇）二丙烯酸酯交联的薄膜组成。将未修饰的微凝胶涂层 PET 盘植入大鼠皮下 4 周，并通过组织学和免疫组织化学分析外植体。微凝胶涂层减少了慢性炎症，并产生了更成熟/组织化的纤维囊。与未修饰的 PET 盘相比，微凝胶涂层样品的纤维胶囊薄了 22%，所含细胞减少了 40%。此外，与未修饰的 PET 对照相比，微凝胶涂层样品含有显着更高水平的巨噬细胞 (80%)。这些结果表明，微凝胶涂层可减少植入生物材料的慢性炎症。 (C) 2010 Wiley periodicals, Inc. J Biomed Mater Res 94A: 252-258, 2010
Inflammatory responses to implanted biomedical devices elicit a foreign body fibrotic reaction that limits device integration and performance in various biomedical applications. We examined chronic inflammatory responses to microgel conformal coatings consisting of thin films of poly(N-isopropylacrylamide) hydrogel microparticles cross-linked with poly(ethylene glycol) diacrylate deposited on poly(ethylene terephthalate) (PET). Unmodified and microgel-coated PET disks were implanted subcutaneously in rats for 4 weeks and explants were analyzed by histology and immunohistochemistry. Microgel coatings reduced chronic inflammation and resulted in a more mature/organized fibrous capsule. Microgel-coated samples exhibited 22% thinner fibrous capsules that contained 40% fewer cells compared to unmodified PET disks. Furthermore, microgel-coated samples contained significantly higher levels of macrophages (80%) than unmodified PET controls. These results demonstrate that microgel coatings reduce chronic inflammation to implanted biomaterials. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res 94A: 252-258, 2010