A highly sensitive signal-amplified gold nanoparticle-based electrochemical immunosensor for dibutyl phthalate detection
A highly sensitive signal-amplified gold nanoparticle-based electrochemical immunosensor for dibutyl phthalate detection
复制标题
用于邻苯二甲酸二丁酯检测的高灵敏度信号放大金纳米粒子电化学免疫传感器
DOI：
10.1016/j.bios.2016.12.007
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发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Zhang, Zhen
中科院分区：
文献类型：
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作者：
Liang, Ya-Ru;Zhang, Zong-Mian;Zhang, Zhen
利用纳米金（AuNPs）诱导信号放大技术，研制了一种简单、信号放大、无标记的邻苯二甲酸二丁酯（DBP）电化学阻抗免疫传感器。该方法基于抗原/壳聚糖/多壁碳纳米管@GONRs/GCE（修饰电极）包被抗原、靶（DBP）和抗DBP抗体的间接竞争结合体系。通过NADH促进的催化沉淀放大AuNP来进行信号放大，这导致AuNP标记的第二抗体与抗DBP偶联后电子转移阻力和静电排斥显着增加。在优化的条件下，该方法的检出限低（7 ng/mL，比使用相同抗体的传统ELISA方法低10倍）;准确度高（回收率为86.0-120.4%），与相应的ELISA方法一致;对某些环境干扰具有较高的耐受性;成本低，样品消耗量低（6 L）。我们的研究结果表明，这种免疫传感器在实际应用中的巨大潜力和高效率的成本效益和敏感的监测在水环境中的DBP。
A simple and signal-amplified, label-free electrochemical impedimetric immunosensor for dibutyl phthalate (DBP), a type of phthalate ester, was developed using gold nanoparticles (AuNPs) induced signal amplification. The approach was based on an indirectly competitive binding system of coating antigen that coated on antigen/chitosan/MWCNTs@GONRs/GCE (modified electrodes), target (DBP) and anti-DBP antibody. Signal amplification was performed via the enlargement of AuNPs through NADH-promoted catalytic precipitation, which caused a marked increase in the electron-transfer resistance and electrostatic repulsion after an AuNP-labeled second antibody conjugated to anti-DBP. Under the optimized conditions, this method displayed: (i) low detection limits (7 ng/mL, 10 times lower than the traditional ELISA method using the same antibody); (ii) satisfactory accuracy (recoveries, 86.0-120.4%) and agreement with corresponding ELISA method; (iii) high tolerance to some environmental interferents; and (iv) low cost and low sample consumption (6 L). Our results demonstrate the great potential and high efficiency of this immunosensor in practical applications for the costeffective and sensitive monitoring of DBP in aquatic environments.