Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation.
Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation.
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DOI：
10.1140/epjti/s40485-015-0016-8
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发表时间：
2015
影响因子：
1
通讯作者：
Notingher I
中科院分区：
文献类型：
--
作者：
Ghita A;Pascut FC;Sottile V;Denning C;Notingher I
干细胞治疗被广泛认为是21世纪的一项关键医学技术，它可能为许多目前无法治愈的疾病提供治疗。这些细胞具有巨大的潜力用于治疗帕金森氏病、糖尿病和心血管疾病等疾病，并在组织工程中作为一种可靠的细胞来源提供移植物来替换和修复病变组织。然而，这一领域的进展一直很困难，部分原因是缺乏能够非侵入性地测量细胞分子特性的技术。这种重复测量可以用来评估干细胞后代分化过程中的培养条件、细胞质量和表型异质性。拉曼光谱是一种基于细胞分子振动引起的激光非弹性散射的光学技术，可以用于提供细胞或细胞器的化学指纹，而不需要固定、裂解或使用标记和其他对比度增强的化学物质。由于分化的细胞专门执行特定的功能，这些细胞产生特定的生物化学物质，可以通过拉曼显微光谱进行检测。本文简要介绍了拉曼显微光谱技术在干细胞体外分化过程中分子特性的研究应用。这篇论文的重点是时间和空间分辨率的拉曼光谱测量，允许在体外重复研究活的干细胞。
Stem cell therapy is widely acknowledged as a key medical technology of the 21st century which may provide treatments for many currently incurable diseases. These cells have an enormous potential for cell replacement therapies to cure diseases such as Parkinson’s disease, diabetes and cardiovascular disorders, as well as in tissue engineering as a reliable cell source for providing grafts to replace and repair diseased tissues. Nevertheless, the progress in this field has been difficult in part because of lack of techniques that can measure non-invasively the molecular properties of cells. Such repeated measurements can be used to evaluate the culture conditions during differentiation, cell quality and phenotype heterogeneity of stem cell progeny. Raman spectroscopy is an optical technique based on inelastic scattering of laser photons by molecular vibrations of cellular molecules and can be used to provide chemical fingerprints of cells or organelles without fixation, lysis or use of labels and other contrast enhancing chemicals. Because differentiated cells are specialized to perform specific functions, these cells produce specific biochemicals that can be detected by Raman micro-spectroscopy. This mini-review paper describes applications of Raman micro-scpectroscopy to measure moleculare properties of stem cells during differentiation in-vitro. The paper focuses on time- and spatially-resolved Raman spectral measurements that allow repeated investigation of live stem cells in-vitro.