The use of human pemphigoid autoantibodies to study the fate of epidermal basal cell hemidesmosomes after trypsin dissociation.
The use of human pemphigoid autoantibodies to study the fate of epidermal basal cell hemidesmosomes after trypsin dissociation.
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使用人类天疱疮自身抗体研究胰蛋白酶解离后表皮基底细胞半桥粒的命运。
DOI：
10.1111/1523-1747.ep12276893
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Diaz,LA
中科院分区：
文献类型：
--
作者：
Takahashi,Y;Mutasim,DF;Patel,HP;Anhalt,GJ;Labib,RS;Diaz,LA
已知在皮肤的胰蛋白酶消化过程中，表皮首先与真皮分离，并且通过表皮细胞间空间的破坏使单个角质形成细胞解离。桥粒单位在细胞间隙水平分离，分裂的桥粒在质膜限制的囊泡中内化;然而，半桥粒在这种条件下的命运尚未研究.我们最近已经表明（Mutasim等：J Invest Dermatol 84：47-53，1985），来自大疱性类天疱疮患者血清的自身抗体在体外结合半桥粒，但不结合桥粒，为这些细胞器提供高度特异性的标记物。利用这些自身抗体，我们研究了半桥粒的命运在胰蛋白酶解离的表皮基底细胞来自新生BALB/c小鼠的皮肤。在胰蛋白酶消化过程中，包括半桥粒在内的质膜的真皮表面部分形成小凹，这些小凹被夹断以产生向核移动的囊泡。这是伴随着收缩的张力细丝远离细胞周边。这种内化过程的机制尚不清楚，但可能涉及细胞骨架的收缩元件。大疱性类天疱疮自身抗体与半桥粒的高度特异性结合可能有助于该细胞器未来的生化和免疫细胞化学研究。
It is known that during trypsinization of the skin, the epidermis is first separated from the dermis and individual keratinocytes are dissociated by disruption of the epidermal intercellular spaces. The desmosomal unit is separated at the level of the intercellular space and the split desmosomes are internalized in plasma membrane- limited vesicles; however the fate of the hemidesmosome under such conditions has not been studied. We have recently shown (Mutasim et al: J Invest Dermatol 84:47–53, 1985) that autoantibodies from the sera of patients with bullous pemphigoid bind in vitro to hemidesmosomes but not to desmosomes providing a highly specific marker for these organelles. Utilizing these autoantibodies, we studied the fate of the hemidesmosome during trypsin dissociation of epidermal basal cells derived from the skin of neonatal BALB/c mice. During trypsinization, portions of the dermal face of the plasma membrane which include hemidesmosomes formed pits which pinched off to produce vesicles that moved toward the nucleus. This was accompanied by retraction of the tonofilaments away from the cell periphery. The mechanism of this internalization process is not yet known, but may involve contractile elements of the cytoskeleton. The highly specific binding of bullous pemphigoid autoantibodies to the hemidesmosome may prove helpful in future biochemical and immunocytochemical studies of this organelle.