An overview of the recent advances on the physiology and molecular biology of lignin peroxidases of Phanerochaete chrysosporium.
An overview of the recent advances on the physiology and molecular biology of lignin peroxidases of Phanerochaete chrysosporium.
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DOI：
10.1016/0168-1656(93)90030-q
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发表时间：
1993-07
影响因子：
4.1
通讯作者：
C. A. Reddy
中科院分区：
文献类型：
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作者：
C. A. Reddy
木质素降解白腐真菌黄孢原毛平革菌 (Phanerochaete chrysosporium) 产生两个胞外过氧化物酶家族，分别称为木质素过氧化物酶 (LIP) 和锰依赖性过氧化物酶 (MNP)，它们是该生物体木质素降解系统的组成部分。产生的 LIP 和 MNP 同工酶的数量和类型随着培养条件的变化而显着变化。蛋白酶介导的 LIP 降解被证明是 P 自发培养物中 LIP 活性衰减的主要原因。金孢子虫。生化突变体的使用不仅提供了有关 LIP 和 MNP 在木质素降解中相对重要性的信息，而且使我们深入了解 LIP 和 MNP 的生产调节。编码主要 LIP 的基因已被克隆和测序，并显示彼此具有高度的同源性。核型分析研究表明，异核菌株含有十条染色体，并且LIP基因分布在至少两条染色体上。
The lignin-degrading white-rot fungusPhanerochaete chrysosporiumproduces two families of extracellular peroxidases designated lignin peroxidases (LIPs) and manganese-dependent peroxidases (MNPs) which are components of the lignin degradation system of this organism. The number and types of LIP and MNP isozymes produced vary dramatically in response to changes in culture conditions. Protease-mediated degradation of LIPs was shown to be the major cause for the decay of LIP activity in idiophasic cultures ofP. chrysosporium. Use of biochemical mutants has not only yielded information on the relative importance of LIPs and MNPs in lignin degradation but has given us insights into the regulation of production of LIPs and MNPs. The genes encoding the major LIPs have been cloned and sequenced and were shown to have a high degree of homology to each other. Karyotyping studies indicated that heterokaryotic strains contain ten chromosomes and that theLIPgenes are distributed on at least two chromosomes.