Thermally Hydrated DPPC Langmuir Film: A Trial Application to the Analysis of Interaction of Sucrose with DPPC Liposome
Thermally Hydrated DPPC Langmuir Film: A Trial Application to the Analysis of Interaction of Sucrose with DPPC Liposome
复制标题
热水合 DPPC Langmuir 膜：蔗糖与 DPPC 脂质体相互作用分析的试验应用
DOI：
10.1021/jp970889e
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发表时间：
1997
影响因子：
3.3
通讯作者：
J. Nishijo
中科院分区：
文献类型：
--
作者：
T. Hasegawa;H. Kawato;and Miwa Toudou;J. Nishijo
研究了热水合二棕榈酰磷脂酰胆碱（DPPC）的Langmuir（L）膜，以确定它是否可以作为DPPC脂质体的适当物理模型。研究了蔗糖与L膜和脂质体的相互作用。没有任何加热过程的常规DPPC L膜显示出由L膜表面上的结合蔗糖引起的膜收缩。随着蔗糖浓度的增加，膜的收缩率增大。发现该收缩影响常规L膜的所有整体特性。另一方面，通过加热过程的水合L膜表明膜膨胀的渗透的蔗糖，虽然在膜中的掺入的水避免了接近蔗糖一点。膨胀随着蔗糖浓度的增加而增加，这一事实与常规L膜的结果相反。比较了水合L膜和脂质体的荧光衰减和荧光各向异性。
The thermally hydrated dipalmitoyl phosphatidyl choline (DPPC) Langmuir (L) film was investigated to determine whether it could be an appropriate physical model of DPPC liposomes. The interaction of sucrose with the L film and the liposome was investigated for the examination. The conventional DPPC L film without any heating process showed a film shrinkage by bound sucrose on the surface of the L film. The film shrinkage was enhanced with an increase of the concentration of sucrose. This shrinkage was found to affect all of the entire characteristics of the conventional L film. On the other hand, the hydrated L film by a heating process indicated a film expansion by the penetrated sucrose, although the incorporated water in the film avoided the approach of sucrose a little. The expansion increased with the concentration of sucrose, a fact that was contrary to the results of the conventional L film. To compare the hydrated L film with liposome, the fluorescence decay and anisotropy of 1,6-diphenyl-1,3,5-he...