Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy.
Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy.
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DOI：
10.1039/c4an00574k
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发表时间：
2014-06-21
期刊：
影响因子：
--
通讯作者：
Haynes CL
中科院分区：
文献类型：
--
作者：
Gunsolus IL;Hu D;Mihai C;Lohse SE;Lee CS;Torelli MD;Hamers RJ;Murhpy CJ;Orr G;Haynes CL
提出了一种与超分辨率荧光显微镜兼容的革兰氏阴性和革兰氏阳性细菌细胞表面荧光染色的方法。该方法利用市售荧光探针标记细胞表面的伯胺。我们证明了两种细菌菌株，革兰氏阴性希瓦氏菌oneidensis MR-1和革兰氏阳性枯草芽孢杆菌168的有效染色。使用结构化照明显微镜和随机光学重建显微镜，这需要高量子产率或专门的染料，我们表明，这种染色方法可以用来解决细菌细胞表面与子衍射极限分辨率。我们进一步使用这种方法来识别纳米材料，特别是硒化镉量子点，与细菌细胞相互作用后的本地化模式。
A method to fluorescently stain the surfaces of both Gram-negative and Gram-positive bacterial cells compatible with super-resolution fluorescence microscopy is presented. This method utilizes a commercially-available fluorescent probe to label primary amines at the surface of the cell. We demonstrate eficient staining of two bacterial strains, the Gram-negative Shewanella oneidensis MR-1 and the Gram-positive Bacillus subtilis 168. Using structured illumination microscopy and stochastic optical reconstruction microscopy, which require high quantum yield or specialized dyes, we show that this staining method may be used to resolve the bacterial cell surface with sub-diffraction-limited resolution. We further use this method to identify localization patterns of nanomaterials, specifically cadmium selenide quantum dots, following interaction with bacterial cells.
DOI：
10.3402/nano.v1i0.5122
发表时间：
2010
期刊：
Nano reviews
影响因子：
--
作者：
Hu D;Orr G
通讯作者：
Orr G