An innovative shape equation to quantify the morphological characteristics of parasitized red blood cells by Plasmodium falciparum and Plasmodium vivax
An innovative shape equation to quantify the morphological characteristics of parasitized red blood cells by Plasmodium falciparum and Plasmodium vivax
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DOI：
10.1177/0954411912474611
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发表时间：
2013-01-01
期刊：
影响因子：
1.8
通讯作者：
Faghihi, Shahab
中科院分区：
文献类型：
--
作者：
Karimi, Alireza;Navidbakhsh, Mahdi;Faghihi, Shahab
红细胞的形态在疟原虫、恶性疟原虫和间日疟原虫的成熟过程中受到显著影响。提出了一种新的形状方程，该方程定义了被恶性疟原虫（Pf-红细胞）和间日疟原虫（Pv-红细胞）寄生的红细胞在感染的四个阶段的形状。使用从健康供体、患有恶性疟原虫和间日疟原虫疟疾的患者收集的血液样品制备吉姆萨染色的薄血膜。分别使用Olysia和Scanning Probe Image Processor软件根据其光学图像测量感染每个阶段的健康红细胞、Pf-red细胞和Pv-red细胞的直径和厚度。利用寄生红细胞的直径和厚度，拟合形状方程，并使用MATHEMATICA绘制相对的二维形状。在环形阶段，Pf-红细胞的形状急剧变化，其厚度增加了82%，而Pv-红细胞保持双凹面（厚度增加了30%）。通过滋养体和随后的鞭毛体阶段，Pf-红细胞完全失去其双凹形状并变成接近球形（直径和厚度类似于8 μ m）。在整个寄生虫发育过程中，PV-红细胞保持双凹面，即使其体积增加。这些结果可能对更快地诊断、预测和治疗人类疟疾和镰状细胞病有实际用途。
The morphology of red blood cells is affected significantly during maturation of malaria parasites, Plasmodium falciparum and Plasmodium vivax. A novel shape equation is presented that defines shape of parasitized red blood cells by P. falciparum (Pf-red blood cells) and P. vivax (Pv-red blood cells) at four stages of infection. The Giemsa-stained thin blood films are prepared using blood samples collected from healthy donors, patients having P. falciparum and P. vivax malaria. The diameter and thickness of healthy red blood cells plus Pf-red blood cells and Pv-red blood cells at each stage of infection are measured from their optical images using Olysia and Scanning Probe Image Processor softwares, respectively. Using diameters and thicknesses of parasitized red blood cells, a shape equation is fitted and relative two-dimensional shapes are plotted using MATHEMATICA. The shape of Pf-red blood cell drastically changes at ring stage as its thickness increases by 82%, while Pv-red blood cell remains biconcave (30% increase in thickness). By trophozoite and subsequent schizont stage, the Pf-red blood cell entirely loses its biconcave shape and becomes near spherical (diameter and thickness of similar to 8 mu m). The Pv-red blood cell remains biconcave throughout the parasite development even though its volume increases. These results could have practical use for faster diagnosis, prediction, and treatment of human malaria and sickle-cell diseases.